LOCUS       sequence-142637-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase
            with C-terminal His6 tag and Glu435>Gln mutant (AarCH6-E435Q).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Mullins EA, Kappock TJ
  TITLE     Crystal structures of Acetobacter aceti succinyl-coenzyme A 
            (CoA):acetate CoA-transferase reveal specificity determinants and 
            illustrate the mechanism used by class I CoA-transferases.
  JOURNAL   Biochemistry. 2012 Oct 23;51(42):8422-34. doi: 10.1021/bi300957f. 
            Epub 2012 Oct 11.
  PUBMED    23030530
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi300957f";
            journalName: "Biochemistry"; date: "2012-10-23- 23"; volume: "51"; 
            issue: "42"; pages: "8422-34"
COMMENT     Sequence Label: pJK516
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..35)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttagcagccg gatctcagtg gtggtggtgg tggtgctcga gtgatgatgg gagcatggtg
       61 cccgtatcaa taaaccgctg atgccaagac agagcttccg tcagcagatg cggtgtgtgc
      121 ttaccaaagg aatttttaag cgcacgatca aaataatcct gcaacatcgg ccggtaatcc
      181 ggatgcgcac atttggaaat aatttcacgc gcacgttgca ccggtgaaag cccacgcaga
      241 tccgccagcc cctgctgggt tacaaaaatc tgcgcatcct gcatgatgtg gtccacatgc
      301 gcagccatcg gcacaatggc tgaaatcttg ccacctttgg ctgtggaggg agacaggaag
      361 atggacaggt aggaactccg ggcaaaatca cccgatcccc caataccatt catcattttg
      421 gaacccatca cgcgggtaga attcacgttt ccgtaaatat ccgcctcaat catgccgttc
      481 atggcaatgc agcctaaacg gcggataatg ccggggctgt tgctgacatc ctgctggcgc
      541 agaatgatct tgctgcggaa gaaatccatc cggttattga tttcttccgc agcttccggg
      601 ctcagcgaga aagacgaggc agaggcaata cgcatacggc cagaatccag catggccaac
      661 atgccatcct gaatcacctc actatagccc accagatttt caaatgggcc ttccttcagc
      721 ccttccagca cggcgttggc cacattgccc acaccagact ggagcggcag aagtgagggc
      781 ggcaaacggt tctgtttgac ttcatgccca aagaaatcaa gcaaatagcc cgcaatagct
      841 tttgctgttt catccggcgc ggcaaacggg gcatttctgt cccggtcatt cgtgcgtaca
      901 atggcagcaa tc
//
