LOCUS       sequence-142667-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Monitor post-translational degradation of Aiolos ZF2 via 
            EGFP:mCherry ratio (Lentiviral, 
            PGK.AiolosAA146-168-10AAFlexLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR
            ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  TITLE     Artichoke
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Aiolos ZF2 (AA146-168) in Cilantro 2
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(169..171,172..684)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(211..232)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(472..491)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cctcgttgac cgaatcaccg acctctctcc ccagggatcg ataccgtcga ctccggaata
       61 gccaccatgt tccagtgtaa tcagtgtggg gcatctttta ctcagaaagg taacctcctc
      121 cgccacatta aactgcacgg cggcggcggc tccggcggcg gcggctccgt gagcaagggc
      181 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      241 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      301 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      361 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
      421 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      481 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      541 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      601 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
      661 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
      721 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
      781 tccgccctga gcaaagaccc
//
