LOCUS       sequence-142669-         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Monitor post-translational degradation of Aiolos AA130-189 via 
            EGFP:mCherry ratio (Lentiviral 
            SFFV.AiolosAA130-189-17AARigidLinker-EGFP.IRES.mCherry).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  TITLE     Artichoke
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Aiolos AA130-189 in Pea
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(325..327,328..840)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(367..388)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(628..647)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggcgcgccag tcctccgaca gactgagtcg gccggtcgaa tcaagcttat cgataccgtc
       61 gactccggaa tagccaccat gtccggattc aatgtcttaa tggttcataa gcgaagccat
      121 actggtgaac gcccattcca gtgtaatcag tgtggggcat cttttactca gaaaggtaac
      181 ctcctccgcc acattaaact gcacacaggg gaaaaacctt ttaagtgtca cctctgcaac
      241 tatgcatgcc aaagaagaga tgcgctcacg cgtgctgaag ctgctgcaaa ggaagctgca
      301 gctaaggagg ctgcagctaa ggctgtgagc aagggcgagg agctgttcac cggggtggtg
      361 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      421 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      481 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
      541 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      601 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      661 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      721 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
      781 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
      841 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      901 gtgctgc
//
