LOCUS       sequence-14267-e        8908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   Senkel S, Waldner C, Ryffel GU, Thomas H
  TITLE     Improved conditional expression systems resulting in physiological 
            level of HNF4alpha expression confirm HNF4alpha induced apoptosis in
            the pancreatic beta-cell line INS-1.
  JOURNAL   BMC Res Notes. 2009 Oct 17;2:210. doi: 10.1186/1756-0500-2-210.
  PUBMED    19835622
REFERENCE   2  (bases 1 to 8908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2009-10-17"; volume: "2:210. doi"; pages: " 
            10.1186/1756-0500-2-210"
COMMENT     Sequence Label: P2_HNF4A8
FEATURES             Location/Qualifiers
     source          1..8908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    2315..2333
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2336..2354
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2364..2388
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2554..2583
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2593..2622
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2632..2661
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2671..2700
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2710..2739
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2773..2802
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4860..4877)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4866..5090
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5223..5242)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5374..5421
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5429..6451
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5429..6451
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6581..6702
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6618..6637)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6672..6691
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6751..6767)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6751..6767)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6764..6786)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6775..6791
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6799..6805,6806..6823,6824..6829))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6844..6865
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6982..6999)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7153..7741)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7233..7252)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7912..8703,8704..8772))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8535..8554
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8773..8877)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtggaaggc
      241 aatgtgagac ctgttgaaat agcacgggct ttggggtcag atgatgctga attccagttc
      301 tgtgttggct ccttcgtagt tatgtgacct catgacgcca cttcatgcag ggggttacgt
      361 accacttaaa tctccagaac attttgggaa attgacatag ggttagcaaa cctcccccac
      421 ccccatataa aaatattaat actttttttt tttctgagat ggagtctggc tctgtcgcca
      481 aggctgcagt gcagtagtgc aatcttggct cactgcaaca tttgcctcct aggttgaaac
      541 gattctcctc cttcagcctc ccgagtagct gagattacag gtgctcgctg ccacacccag
      601 ctaatttttg tatttttagt agagatggga ttacaccgtg ttggccagcc tggtctcaaa
      661 ctcctgacct caagtgatcc acctgcctca gcctcccaaa ctgctgggat tacaggcatg
      721 agccactaca cccgaccaag aatattaata ctttttttta aaaaatatcg aagttattta
      781 tttatttatt tatttattta tttatttatt tattttgaga tggagtctct ctctgttgcc
      841 caggctggag tgcagtggca cgatcttggc tcactgcaac ctctgtcccc ctggttcaaa
      901 cgattctctt gcctcagcct cccgtttgcc accatgcccg gctaattttt ttatttttgg
      961 tagagacggg gtttcaccat gttggtcagg ctggcctcaa actcctgacc tcgtgatcgg
     1021 tgcgcctcgg cctcccaaaa tgctggggtt acaggcgtga gccactgcac ccggccccaa
     1081 aatctaacgt ttgttatcac tgttgtttta gaaaagaaag gacattttaa tgcaacccat
     1141 gcccatctgt aggggctgaa ggtccctctg aggtgattga taaagctgaa aaaagagact
     1201 atgggaagag aagaggaata ttatcaatta gctattttag aagcagggca aactatgagc
     1261 taggtattcc tataaggaaa tgaaataatt tactaaaaaa catacagcaa gaaaataata
     1321 gagctggagt tttctgattt ttctgagtcc agaatttgtt tcactgttct catttcagct
     1381 ctctctaaca ggatgaatga ccttgatttc agaatcccca agcaggtggt gagatccaaa
     1441 actgagacaa aagaaacggg gctgttccaa aaaaaaagct aggtggcagg tgtctaacat
     1501 gccagggagc taaaacagag tgtgtgagtt tcagcagcag gttgaattta gaatggggaa
     1561 ggagaccaga ggagacgcca gacaggatga ctttgtccca ttggcctgga ggcagcccca
     1621 tgtttctcca cccctcatat cactcaccag tttgtaatag tatctttgaa tgacgatctg
     1681 attaaggtcc gtctcctcca ttagtccaca agtttcgggg gtacatctac tttgctcatt
     1741 tccatatccc cagagtctag cacaaggcct ggtacatagt aggtgctcaa taaatatgtt
     1801 agatgaaagg aagataacac ctctatgtac tagcagtgag actccaggca tgcaatttct
     1861 ctctgtcctt cagtcccttc atctcaaggt ttaatttaaa tatggtaacg cctgtatgca
     1921 actcccagca tccagtaggc actcactaaa cacagttctc caccctcctt ttttcctctg
     1981 cccctccctc ggttttccca ctacttcctg catggtgaca cacccatagt ttggagccat
     2041 aaaacccaac ccaggttgga ctctcacctc tccagcccct tctgctccgg ccctgtcctc
     2101 aaattggggg gctgatgtcc ccatacacct ggctctgggt tcccctaacc ccagagtgca
     2161 ggactaggac ccgagtggac ctcaggtctg gccaggtcgc cattgccatg gagacagcaa
     2221 cagtccccag ccgcgggttc cctaagtgac tggttactct ttaacgtatc cacccacctt
     2281 gggtgattag aagaatcaat aagataaacg cgtctcccta tcagtgatag agatctccct
     2341 atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     2401 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccggactcta
     2461 gcgtttaaac ttaagcttgg taccgagctc ggatccacta gtccagtgtg gtggaattct
     2521 gcagatatcc agcacagtgg cggccgcgct atggagcaaa agctcatttc tgaagaggac
     2581 ttgaatgaaa tggagcaaaa gctcatttct gaagaggact tgaatgaaat ggagcaaaag
     2641 ctcatttctg aagaggactt gaatgaaatg gagcaaaagc tcatttctga agaggacttg
     2701 aatgaaatgg agcaaaagct catttctgaa gaggacttga atgaaatgga gagcttgggc
     2761 gacctcacca tggagcaaaa gctcatttct gaagaggact tgaattcaag gcctggtacc
     2821 atggtcagcg tgaacgcgcc cctcggggct ccagtggaga gttcttacga cacgtcccca
     2881 tcagaaggca ccaacctcaa cgcgcccaac agcctgggtg tcagcgccct gtgtgccatc
     2941 tgcggggacc gggccacggg caaacactac ggtgcctcga gctgtgacgg ctgcaagggc
     3001 ttcttccgga ggagcgtgcg gaagaaccac atgtactcct gcagatttag ccggcagtgc
     3061 gtggtggaca aagacaagag gaaccagtgc cgctactgca ggctcaagaa atgcttccgg
     3121 gctggcatga agaaggaagc cgtccagaat gagcgggacc ggatcagcac tcgaaggtca
     3181 agctatgagg acagcagcct gccctccatc aatgcgctcc tgcaggcgga ggtcctgtcc
     3241 cgacagatca cctcccccgt ctccgggatc aacggcgaca ttcgggcgaa gaagattgcc
     3301 agcatcgcag atgtgtgtga gtccatgaag gagcagctgc tggttctcgt tgagtgggcc
     3361 aagtacatcc cagctttctg cgagctcccc ctggacgacc aggtggccct gctcagagcc
     3421 catgctggcg agcacctgct gctcggagcc accaagagat ccatggtgtt caaggacgtg
     3481 ctgctcctag gcaatgacta cattgtccct cggcactgcc cggagctggc ggagatgagc
     3541 cgggtgtcca tacgcatcct tgacgagctg gtgctgccct tccaggagct gcagatcgat
     3601 gacaatgagt atgcctacct caaagccatc atcttctttg acccagatgc caaggggctg
     3661 agcgatccag ggaagatcaa gcggctgcgt tcccaggtgc aggtgagctt ggaggactac
     3721 atcaacgacc gccagtatga ctcgcgtggc cgctttggag agctgctgct gctgctgccc
     3781 accttgcaga gcatcacgtg gcagatgatc gagcagatcc agttcatcaa gctcttcggc
     3841 atggccaaga ttgacaacct gttgcaggag atgctgctgg gagggtcccc cagcgatgca
     3901 ccccatgccc accaccccct gcaccctcac ctgatgcagg aacatatggg aaccaacgtc
     3961 atcgttgcca acacaatgcc cactcacctc agcaacggac agatgtgtga gtggccccga
     4021 cccaggggac aggcagccac ccctgagacc ccacagccct caccgccagg tggctcaggg
     4081 tctgagccct ataagctcct gccgggagcc gtcgccacaa tcgtcaagcc cctctctgcc
     4141 atcccccagc cgaccatcac caagcaggaa gttatctagc aagccgctgg ggcttggggg
     4201 ctccactggc tccccccagc cccctaagag agcacctggt gatcacgtgg tcacggcaaa
     4261 ggaagacgtg atgccaggac cagtcccaga gcaggaatgg gaaggatgaa gggcccgaga
     4321 acatggccta agggccacat cccactgcca cccttgacgc cctgctctgg ataacaagac
     4381 tttgacttgg ggagacctct actgccttgg acaacttttc tcatgttgaa gccactgcct
     4441 tcaccttcac cttcatccat gtccaacccc cgacttcatc ccaaaggaca gccgcctgga
     4501 gatgacttga ggccttactt aaacccagct cccttcttcc ctagcctggt gcttctcctc
     4561 tcctagcccc ggtcatggtg tccagacaga gccctgtgag gctgggtcca attgtggcac
     4621 ttggggcacc ttgctcctcc ttctgctgct gcccccacct ctgctgcctc cctctgctgt
     4681 caccttgctc agccatcccg tcttctccaa caccacctct acagaggcca aggaggcctt
     4741 ggaaacgatt cccccagtca ttctgggaac atgttgtaag cactgactgg gaccaggcac
     4801 caggcagggt ctagagcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc
     4861 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     4921 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     4981 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     5041 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     5101 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     5161 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     5221 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     5281 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     5341 aaaacttgat tagggtgatg gttcacgtac ctagaagttc ctattccgaa gttcctattc
     5401 tctagaaagt ataggaactt ccttggccaa aaagcctgaa ctcaccgcga cgtctgtcga
     5461 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
     5521 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
     5581 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
     5641 cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc
     5701 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
     5761 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
     5821 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     5881 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     5941 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     6001 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     6061 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     6121 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     6181 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
     6241 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     6301 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     6361 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     6421 ccccagcact cgtccgaggg caaaggaata gcacgtacta cgagatttcg attccaccgc
     6481 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     6541 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     6601 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6661 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     6721 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     6781 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     6841 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     6901 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     6961 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7021 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7081 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7141 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7201 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7261 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7321 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7381 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7441 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7501 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7561 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7621 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7681 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7741 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     7801 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     7861 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     7921 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     7981 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8041 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8101 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8161 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8221 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8281 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8341 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8401 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8461 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8521 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8581 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8641 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     8701 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     8761 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     8821 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     8881 atttccccga aaagtgccac ctgacgtc
//
