LOCUS       sequence-142677-         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 Est14, C-terminal His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 hydrolase/transferase enzymes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK409
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..35)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(861..872)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttagcagccg gatctcagtg gtggtggtgg tggtgctcga gcagattcaa tgtgcgttta
       61 agtgctgcac agtaggcatc cataaacctg cgggaaagtt cggcatctgg cacatacata
      121 tcaaacacat gatacgcacc cggcaccacc ataacttccg ttggcacggc cgcagccatc
      181 aggcgcgttg cgtaatcaag gtcttctttc aaaaacagat ccattgatcc cacaccaata
      241 aaggtgggag gcaaacctgc cagatcagtt gcacggccag cggctgcata tggtggtgtt
      301 tgtgggccgc cagctttatc acccagataa gcctgccatg cgtatttgtt ggcagaacgt
      361 gtccagacaa attcgccaaa attggcgccc gggtcactgt ttattgccgt gcgatcatcc
      421 agcatggggt aaataagcaa ctgaaaagcc agtttgtatt cttttttgtc acgcgccatc
      481 aggctcagcg ctgcacttaa accaccacct gcactttcac ccgcaacaac aaccttatca
      541 cgcgaaatac caagttttct ggcgttttca tgcacccatt tcagcactgc gtaacagtct
      601 tcaattgcac cgggatattt gacctcaggc gccagccgat aatctatcga gaaaatctga
      661 aagcccagat cactagccag cttttggcat ttggggtcat ccacttctgg atggccagaa
      721 acaagaccgc caccatgaat ataaaccata gctggcgcat tgggctgcac ttcacgcggc
      781 gtataaacaa ccacacgcac atctggggca ttgttcaacc ccggtacgta ttcttcccgc
      841 gtgataacat ctggcagagg ccgaccttct ataaggcgca cagatgttag aaaaacttct
      901 cgcacttcag gaagtgaaag cgacgaaaga ccatcaaaag atggcatttt ctcaagaatg
      961 g
//
