LOCUS       sequence-142678-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 hypothetical acetic acid resistance 
            protein B, C-terminal His6 tag (AaAarBH6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 acetic acid resistance (aar) gene 
            constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK103
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             500..517
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(570..588)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      584..631
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(755..774)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgctgggt aaaccgcagt tctttggtct
       61 gcgcgttgct cgcatgtgtg cgaacgcatg tttcggtcac cgtactttcg catctgttct
      121 gtatctggcc acctttcgtt accgtacctt ccagaagcag cagcagcgtc tcgaacgcat
      181 tccggatcca gctcactggc cagcgtgcct gaccgacgca ccggctcgta aataccgtgg
      241 ctatgcacct ccgtccgcgc tgtgtggtct gaatcgtgaa cgtgctgagt tcgcgccgac
      301 taaactgcag gcatggcgtc gcaaatacct gcataacatc cgtgcatggg aaggcggtat
      361 gccggtgccg ccgcaacgtc tgccggaatc tgcggtatgc gcactgcccg ggtacatcaa
      421 atggtaccgc gcgcagtgcc gtgcactgcc ggccagcgct cactccgctg ttccgccgct
      481 ggcactgcgt gctctcgagc accaccacca ccaccactga gatccggctg ctaacaaagc
      541 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
      601 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc
      661 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
      721 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
      781 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
      841 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
      901 a
//
