LOCUS       sequence-142679-        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 hypothetical acetic acid resistance 
            protein B, C-terminal His6 tag (AaAarBH6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 acetic acid resistance (aar) gene 
            constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK103
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..43)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             519..541
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(572..591)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(573..591)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgaga gcacgcagtg
       61 ccagcggcgg aacagcggag tgagcgctgg ccggcagtgc acggcactgc gcgcggtacc
      121 atttgatgta cccgggcagt gcgcataccg cagattccgg cagacgttgc ggcggcaccg
      181 gcataccgcc ttcccatgca cggatgttat gcaggtattt gcgacgccat gcctgcagtt
      241 tagtcggcgc gaactcagca cgttcacgat tcagaccaca cagcgcggac ggaggtgcat
      301 agccacggta tttacgagcc ggtgcgtcgg tcaggcacgc tggccagtga gctggatccg
      361 gaatgcgttc gagacgctgc tgctgcttct ggaaggtacg gtaacgaaag gtggccagat
      421 acagaacaga tgcgaaagta cggtgaccga aacatgcgtt cgcacacatg cgagcaacgc
      481 gcagaccaaa gaactgcggt ttacccagca tatgtatatc tccttcttaa agttaaacaa
      541 aattatttct agagggaaac cgttgtggtc tccctatagt gagtcgtatt aatttcgcgg
      601 gatcgagatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc
      661 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat
      721 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg
      781 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac
      841 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc
      901 tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca
      961 taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac
     1021 ccgtatcgtg agcatcctct ctcgtttcat cgg
//
