LOCUS       sequence-14269-e        9199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9199)
  AUTHORS   Senkel S, Waldner C, Ryffel GU, Thomas H
  TITLE     Improved conditional expression systems resulting in physiological 
            level of HNF4alpha expression confirm HNF4alpha induced apoptosis in
            the pancreatic beta-cell line INS-1.
  JOURNAL   BMC Res Notes. 2009 Oct 17;2:210. doi: 10.1186/1756-0500-2-210.
  PUBMED    19835622
REFERENCE   2  (bases 1 to 9199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2009-10-17"; volume: "2:210. doi"; pages: " 
            10.1186/1756-0500-2-210"
COMMENT     Sequence Label: P2_DD-HNF4A8_C106R
FEATURES             Location/Qualifiers
     source          1..9199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    2315..2333
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2336..2354
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2364..2388
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2497..2506
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2503..2505,2506..2826)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             2842..2871
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2881..2910
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2920..2949
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2959..2988
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2998..3027
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3061..3090
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(5151..5168)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5157..5381
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5514..5533)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5665..5712
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5720..6742
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5720..6742
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6872..6993
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6909..6928)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6963..6982
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7042..7058)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7042..7058)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7055..7077)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7066..7082
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7090..7096,7097..7114,7115..7120))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7135..7156
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7273..7290)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7444..8032)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7524..7543)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8203..8994,8995..9063))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8826..8845
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9064..9168)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtggaaggc
      241 aatgtgagac ctgttgaaat agcacgggct ttggggtcag atgatgctga attccagttc
      301 tgtgttggct ccttcgtagt tatgtgacct catgacgcca cttcatgcag ggggttacgt
      361 accacttaaa tctccagaac attttgggaa attgacatag ggttagcaaa cctcccccac
      421 ccccatataa aaatattaat actttttttt tttctgagat ggagtctggc tctgtcgcca
      481 aggctgcagt gcagtagtgc aatcttggct cactgcaaca tttgcctcct aggttgaaac
      541 gattctcctc cttcagcctc ccgagtagct gagattacag gtgctcgctg ccacacccag
      601 ctaatttttg tatttttagt agagatggga ttacaccgtg ttggccagcc tggtctcaaa
      661 ctcctgacct caagtgatcc acctgcctca gcctcccaaa ctgctgggat tacaggcatg
      721 agccactaca cccgaccaag aatattaata ctttttttta aaaaatatcg aagttattta
      781 tttatttatt tatttattta tttatttatt tattttgaga tggagtctct ctctgttgcc
      841 caggctggag tgcagtggca cgatcttggc tcactgcaac ctctgtcccc ctggttcaaa
      901 cgattctctt gcctcagcct cccgtttgcc accatgcccg gctaattttt ttatttttgg
      961 tagagacggg gtttcaccat gttggtcagg ctggcctcaa actcctgacc tcgtgatcgg
     1021 tgcgcctcgg cctcccaaaa tgctggggtt acaggcgtga gccactgcac ccggccccaa
     1081 aatctaacgt ttgttatcac tgttgtttta gaaaagaaag gacattttaa tgcaacccat
     1141 gcccatctgt aggggctgaa ggtccctctg aggtgattga taaagctgaa aaaagagact
     1201 atgggaagag aagaggaata ttatcaatta gctattttag aagcagggca aactatgagc
     1261 taggtattcc tataaggaaa tgaaataatt tactaaaaaa catacagcaa gaaaataata
     1321 gagctggagt tttctgattt ttctgagtcc agaatttgtt tcactgttct catttcagct
     1381 ctctctaaca ggatgaatga ccttgatttc agaatcccca agcaggtggt gagatccaaa
     1441 actgagacaa aagaaacggg gctgttccaa aaaaaaagct aggtggcagg tgtctaacat
     1501 gccagggagc taaaacagag tgtgtgagtt tcagcagcag gttgaattta gaatggggaa
     1561 ggagaccaga ggagacgcca gacaggatga ctttgtccca ttggcctgga ggcagcccca
     1621 tgtttctcca cccctcatat cactcaccag tttgtaatag tatctttgaa tgacgatctg
     1681 attaaggtcc gtctcctcca ttagtccaca agtttcgggg gtacatctac tttgctcatt
     1741 tccatatccc cagagtctag cacaaggcct ggtacatagt aggtgctcaa taaatatgtt
     1801 agatgaaagg aagataacac ctctatgtac tagcagtgag actccaggca tgcaatttct
     1861 ctctgtcctt cagtcccttc atctcaaggt ttaatttaaa tatggtaacg cctgtatgca
     1921 actcccagca tccagtaggc actcactaaa cacagttctc caccctcctt ttttcctctg
     1981 cccctccctc ggttttccca ctacttcctg catggtgaca cacccatagt ttggagccat
     2041 aaaacccaac ccaggttgga ctctcacctc tccagcccct tctgctccgg ccctgtcctc
     2101 aaattggggg gctgatgtcc ccatacacct ggctctgggt tcccctaacc ccagagtgca
     2161 ggactaggac ccgagtggac ctcaggtctg gccaggtcgc cattgccatg gagacagcaa
     2221 cagtccccag ccgcgggttc cctaagtgac tggttactct ttaacgtatc cacccacctt
     2281 gggtgattag aagaatcaat aagataaacg cgtctcccta tcagtgatag agatctccct
     2341 atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     2401 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccggactcta
     2461 gcgtttaaac ttaagcttgg taccgagctc ggatccgcca ccatgggagt gcaggtggaa
     2521 accatctccc caggagacgg gcgcaccttc cccaagcgcg gccagacctg tgtggtgcac
     2581 tacaccggga tgcttgaaga tggaaagaaa gtcgattcct cccgggacag aaacaagccc
     2641 tttaagttta tgctaggcaa gcaggaggtg atccgaggct gggaagaagg ggttgcccag
     2701 atgagtgtgg gtcagagagc caaactgact atatctccag attatgccta tggtgccact
     2761 gggcacccag gcatcatccc accacatgcc actctcgtct tcgatgtgga gcttctaaaa
     2821 ccggaagaat tgcgcgctat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg
     2881 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa
     2941 gaggacttga atgaaatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag
     3001 caaaagctca tttctgaaga ggacttgaat gaaatggaga gcttgggcga cctcaccatg
     3061 gagcaaaagc tcatttctga agaggacttg aattcaaggc ctggtaccca attcgtcagc
     3121 gtgaacgcgc ccctcggggc tccagtggag agttcttacg acacgtcccc atcagaaggc
     3181 accaacctca acgcgcccaa cagcctgggt gtcagcgccc tgtgtgccat ctgcggggac
     3241 cgggccacgg gcaaacacta cggtgcctcg agctgtgacg gctgcaaggg cttcttccgg
     3301 aggagcgtgc ggaagaacca catgtactcc tgcagattta gccggcagtg cgtggtggac
     3361 aaagacaaga ggaaccagtg ccgctaccgc aggctcaaga aatgcttccg ggctggcatg
     3421 aagaaggaag ccgtccagaa tgagcgggac cggatcagca ctcgaaggtc aagctatgag
     3481 gacagcagcc tgccctccat caatgcgctc ctgcaggcgg aggtcctgtc ccgacagatc
     3541 acctcccccg tctccgggat caacggcgac attcgggcga agaagattgc cagcatcgca
     3601 gatgtgtgtg agtccatgaa ggagcagctg ctggttctcg ttgagtgggc caagtacatc
     3661 ccagctttct gcgagctccc cctggacgac caggtggccc tgctcagagc ccatgctggc
     3721 gagcacctgc tgctcggagc caccaagaga tccatggtgt tcaaggacgt gctgctccta
     3781 ggcaatgact acattgtccc tcggcactgc ccggagctgg cggagatgag ccgggtgtcc
     3841 atacgcatcc ttgacgagct ggtgctgccc ttccaggagc tgcagatcga tgacaatgag
     3901 tatgcctacc tcaaagccat catcttcttt gacccagatg ccaaggggct gagcgatcca
     3961 gggaagatca agcggctgcg ttcccaggtg caggtgagct tggaggacta catcaacgac
     4021 cgccagtatg actcgcgtgg ccgctttgga gagctgctgc tgctgctgcc caccttgcag
     4081 agcatcacgt ggcagatgat cgagcagatc cagttcatca agctcttcgg catggccaag
     4141 attgacaacc tgttgcagga gatgctgctg ggagggtccc ccagcgatgc accccatgcc
     4201 caccaccccc tgcaccctca cctgatgcag gaacatatgg gaaccaacgt catcgttgcc
     4261 aacacaatgc ccactcacct cagcaacgga cagatgtgtg agtggccccg acccagggga
     4321 caggcagcca cccctgagac cccacagccc tcaccgccag gtggctcagg gtctgagccc
     4381 tataagctcc tgccgggagc cgtcgccaca atcgtcaagc ccctctctgc catcccccag
     4441 ccgaccatca ccaagcagga agttatctag caagccgctg gggcttgggg gctccactgg
     4501 ctccccccag ccccctaaga gagcacctgg tgatcacgtg gtcacggcaa aggaagacgt
     4561 gatgccagga ccagtcccag agcaggaatg ggaaggatga agggcccgag aacatggcct
     4621 aagggccaca tcccactgcc acccttgacg ccctgctctg gataacaaga ctttgacttg
     4681 gggagacctc tactgccttg gacaactttt ctcatgttga agccactgcc ttcaccttca
     4741 ccttcatcca tgtccaaccc ccgacttcat cccaaaggac agccgcctgg agatgacttg
     4801 aggccttact taaacccagc tcccttcttc cctagcctgg tgcttctcct ctcctagccc
     4861 cggtcatggt gtccagacag agccctgtga ggctgggtcc aattgtggca cttggggcac
     4921 cttgctcctc cttctgctgc tgcccccacc tctgctgcct ccctctgctg tcaccttgct
     4981 cagccatccc gtcttctcca acaccacctc tacagaggcc aaggaggcct tggaaacgat
     5041 tcccccagtc attctgggaa catgttgtaa gcactgactg ggaccaggca ccaggcaggg
     5101 tctagagcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
     5161 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     5221 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     5281 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     5341 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     5401 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     5461 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5521 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5581 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5641 ttagggtgat ggttcacgta cctagaagtt cctattccga agttcctatt ctctagaaag
     5701 tataggaact tccttggcca aaaagcctga actcaccgcg acgtctgtcg agaagtttct
     5761 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
     5821 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
     5881 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
     5941 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc
     6001 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
     6061 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     6121 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
     6181 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
     6241 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     6301 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
     6361 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     6421 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
     6481 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
     6541 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
     6601 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
     6661 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
     6721 tcgtccgagg gcaaaggaat agcacgtact acgagatttc gattccaccg ccgccttcta
     6781 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     6841 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     6901 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6961 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     7021 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7081 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7141 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7201 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7261 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7321 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7381 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7441 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7501 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7561 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7621 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7681 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7741 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7801 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7861 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7921 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7981 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8041 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8101 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8161 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8221 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8281 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8341 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8401 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8461 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8521 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8581 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8641 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8701 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8761 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8821 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8881 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8941 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9001 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9061 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9121 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9181 aaaagtgcca cctgacgtc
//
