LOCUS       sequence-142691-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ratiometric pHluorin2 with a gpi linkage sequence in a middle entry 
            clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Stawicki TM, Owens KN, Linbo T, Reinhart KE, Rubel EW, Raible DW
  TITLE     The zebrafish merovingian mutant reveals a role for pH regulation in
            hair cell toxicity and function.
  JOURNAL   Dis Model Mech. 2014 Jul;7(7):847-56. doi: 10.1242/dmm.016576.
  PUBMED    24973752
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dmm"; 
            journalName: "Dis Model Mech"; date: "2014-07"; volume: "7"; issue: 
            "7"; pages: "847-56"
COMMENT     Sequence Label: pME phluorin2-gpi
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(392..419)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(511..597)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     646..668
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     660..677
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     661..677
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    693..792
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      241 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      301 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
      361 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
      421 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
      481 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
      541 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
      601 cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt
      661 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
      721 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      781 aaaaagcagg ctaaatggct cagcggatga caacacagct gctgctcctt ctagtgtggg
      841 tggctgtagt aggggaggct cagacaagga ttgcaatggt gagcaagggc gaggagctgt
      901 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga c
//
