LOCUS       sequence-142705-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses NET1A-V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE,
            Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
  TITLE     Identification of AMPK Phosphorylation Sites Reveals a Network of 
            Proteins Involved in Cell Invasion and Facilitates Large-Scale 
            Substrate Prediction.
  JOURNAL   Cell Metab. 2015 Nov 3;22(5):907-21. doi: 
            10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8.
  PUBMED    26456332
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.09.009"; journalName: "Cell Metab"; date: 
            "2015-11-3- 3"; volume: "22"; issue: "5"; pages: "907-21"
COMMENT     Sequence Label: pcDNA 3.2/V5-DEST NET1A V5 WT
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    94..118
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agttaagcta tcaacaagtt tgtacaaaaa agttggcatg
      121 gtggcacatg atgagactgg aggtctccta cctattaaaa ggaccatacg agtcctagat
      181 gtcaataacc agtccttcag agaacaagag gagccaagca ataaaagagt tcgacctctg
      241 gctcgtgtca cgtccttggc aaatttaatc tctcctgtaa gaaatggagc tgtcagacgt
      301 tttggtcaaa caatacagtc atttaccctt cgtggtgacc acagatcccc agcctctgcc
      361 cagaagtttt ctagcaggtc aacagtccca acacccgcca agagaaggag cagtgcactg
      421 tggtcagaga tgctggacat caccatgaag gagtctctca ccaccaggga gatcagacgg
      481 caggaggcaa tatatgaaat gtcccgaggt gaacaggatt taattgagga tctcaaactt
      541 gcaagaaagg cctaccatga ccccatgtta aagttgtcca tcatgtcaga agaggaactc
      601 acacatatat ttggtgatct ggactcttac atacctctgc atgaagattt gttgacaaga
      661 ataggagaag caaccaagcc tgatggaaca gtggagcaga ttggtcacat tctcgtgagc
      721 tggttaccgc gcttgaatgc ctacagaggt tactgtagta accagctggc agccaaagct
      781 cttcttgatc aaaagaaaca ggatccaaga gtccaagact tcctccagcg atgtctcgag
      841 tctcccttca gtcgaaaact agatctttgg agtttcctag atatccctcg aagtcgccta
      901 gtcaaatacc ctttact
//
