LOCUS       sequence-142718-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  an alpha-crystallin::Venus (green lens) reporter cassette flanked by
            FRT sites and with a single loxP site at the 3' end was inserted in 
            the middle of pKHR4 mcs. Genbank accession number is KU144823..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Hoshijima K, Jurynec MJ, Grunwald DJ
  TITLE     Precise Editing of the Zebrafish Genome Made Simple and Efficient.
  JOURNAL   Dev Cell. 2016 Mar 21;36(6):654-67. doi: 
            10.1016/j.devcel.2016.02.015.
  PUBMED    27003937
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2016.02"; journalName: "Dev Cell"; date: 
            "2016-03-21- 21"; volume: "36"; issue: "6"; pages: "654-67"
COMMENT     Sequence Label: pKHR5
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     65..81
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     65..81
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    101..134
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    198..332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(235..254)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     289..308
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    369..576
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     565..582
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(603..854)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(645..666)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgcggtggcg gccaggtcct ttagtcgggt attcgggtag ggataacagg gtaatggcgg
       61 ccgctctaga actagtggat cccccgggct gcaggaattc gaagttccta ttctctagaa
      121 agtataggaa cttcttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
      181 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
      241 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
      301 aactcatcaa tgtatcttat catgtctgga tcatcatcga tggtacccac cgcggtggcg
      361 gcgatctctc cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc
      421 ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat
      481 tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg
      541 ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag cgggccgctt
      601 tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg
      661 accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactggta gctcaggtag
      721 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
      781 gcgagctgca cgccgccgtc ctcgatgttg tggcggatct tgaagttggc cttgatgccg
      841 ttcttctgct tgtcggcggt gatatagacg ttgtggctgt tgtagtt
//
