LOCUS       sequence-142720-         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  an alpha-crystallin::Venus (green lens) reporter cassette flanked by
            FRT sites and with a single loxP site at the 3' end was inserted in 
            the middle of pKHR4 mcs. Genbank accession number is KU144823..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Hoshijima K, Jurynec MJ, Grunwald DJ
  TITLE     Precise Editing of the Zebrafish Genome Made Simple and Efficient.
  JOURNAL   Dev Cell. 2016 Mar 21;36(6):654-67. doi: 
            10.1016/j.devcel.2016.02.015.
  PUBMED    27003937
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2016.02"; journalName: "Dev Cell"; date: 
            "2016-03-21- 21"; volume: "36"; issue: "6"; pages: "654-67"
COMMENT     Sequence Label: pKHR5
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..65
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     50..66
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    81..114
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(116..149)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(760..781)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cggtccttta gtcgggtatt cgggtaggga taacagggta atggcggctc gaggtcgacg
       61 gtatcgataa gcttgatatc ataacttcgt ataatgtatg ctatacgaag ttattgaagt
      121 tcctatactt tctagagaat aggaacttca ttaatagtgt gcattcagtg caggatgtta
      181 tgtatttatt ttctgtagtg caaacttttt caaagaattt tccagtctaa gcttttcagt
      241 caagaaaaaa aaaaagcgat tgattatctg tgatatttaa aaaaattagc tcttaaatct
      301 ggtgaaccaa gtggccacaa cacattaaat actgacgttc tacagccatg tagcttcatc
      361 tggtctggtt tgttttggca ggcacttatt ttccggtgat agttggccac aagagtattg
      421 tctgagccat tcagtgctag actgtcattc tcaggtcaga gtccaccccg ctgattctgc
      481 tgacaacact gttcccacca tgagataatg ccattccaga gagatccatt tgtaagcccc
      541 tctttctgca gcacaggtat ataaccaggg gtctgcctcc actaaggccg gcacacatca
      601 tttggggatc tttgtactgt actagcctct attagcctcc tacttgcgtt cagttcacat
      661 cttttgttca tcaggtgcaa taagttacag taaggagtta ccaggtctga caacatggtg
      721 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      781 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      841 ctgaccctga agctgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      901 accaccctgg gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
      961 g
//
