LOCUS       sequence-142724-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FKBP1B
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             478..495
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(548..566)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      562..609
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(733..752)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccat gggcgtggag atcgagacca tctcccccgg
      121 agacggaagg acattcccca agaagggcca aacgtgtgtg gtgcactaca caggaatgct
      181 ccaaaatggg aagaagtttg attcatccag agacagaaac aaacctttca agttcagaat
      241 tggcaaacag gaagtcatca aaggttttga agagggtgca gcccagatga gcttggggca
      301 gagggcgaag ctgacctgca cccctgatgt ggcatatgga gccacgggcc accccggtgt
      361 catccctccc aatgccaccc tcatctttga cgtggagctg ctcaacttag agtgacagta
      421 aaggtggata cggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc actcgagcac
      481 caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
      541 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
      601 ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg
      661 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
      721 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
      781 cccgtca
//
