LOCUS       sequence-142725-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CDC42EP1-V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE,
            Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
  TITLE     Identification of AMPK Phosphorylation Sites Reveals a Network of 
            Proteins Involved in Cell Invasion and Facilitates Large-Scale 
            Substrate Prediction.
  JOURNAL   Cell Metab. 2015 Nov 3;22(5):907-21. doi: 
            10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8.
  PUBMED    26456332
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.09.009"; journalName: "Cell Metab"; date: 
            "2015-11-3- 3"; volume: "22"; issue: "5"; pages: "907-21"
COMMENT     Sequence Label: pcDNA 3.2/V5-DEST CDC42EP1 WT
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagttaagct atcaacaagt ttgtacaaaa aagttggcat
      121 gcccggcccc caggggggca gaggcgccgc caccatgagc ctgggcaagc tctcgcctgt
      181 gggctgggtg tccagttcac agggaaagag gcggctgact gcagacatga tcagccaccc
      241 actcggggac ttccgccaca ccatgcatgt gggccgtggc ggggatgtct tcggggacac
      301 gtccttcctc agcaaccacg gtggcagctc cgggagcacc catcgctcac cccgcagctt
      361 cctggccaag aagctgcagc tggtgcggag ggtgggggcg cccccccgga ggatggcatc
      421 tccccctgca ccctccccgg ctccaccggc catctccccc atcatcaaga acgccatctc
      481 cctgccccag ctcaaccagg ccgcctacga cagcctcgtg gttggcaagc tcagcttcga
      541 cagcagcccc accagctcca cggacggcca ctccagctac ggcctggact ctgggttctg
      601 caccatctcc cgcctgcccc gctcggaaaa gccgcatgac cgagaccggg atggttcctt
      661 cccctctgag cccgggcttc gccgctctga ctctctcttg tccttccgcc tggacctcga
      721 ccttgggccc tcactcctca gcgagctgct aggggtcatg agcctgccag aagcccctgc
      781 agctgagact ccagcccccg ctgcaaaccc cccagcccct actgcaaacc ccacgggtcc
      841 tgctgcaaac cccccagcca ctactgcaaa ccccc
//
