LOCUS       sequence-142726-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FKBP1B
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(424..444)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(469..486)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             497..519
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    534..558
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(558..577)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(559..577)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(661..680)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga
       61 cggagctcga attcggatcc gtatccacct ttactgtcac tctaagttga gcagctccac
      121 gtcaaagatg agggtggcat tgggagggat gacaccgggg tggcccgtgg ctccatatgc
      181 cacatcaggg gtgcaggtca gcttcgccct ctgccccaag ctcatctggg ctgcaccctc
      241 ttcaaaacct ttgatgactt cctgtttgcc aattctgaac ttgaaaggtt tgtttctgtc
      301 tctggatgaa tcaaacttct tcccattttg gagcattcct gtgtagtgca ccacacacgt
      361 ttggcccttc ttggggaatg tccttccgtc tccgggggag atggtctcga tctccacgcc
      421 catggattgg aagtacaggt tctcggtacc cagatctaca ccagaagaat gatgatgatg
      481 atggtgcata tgtatatctc cttcttaaag ttaaacaaaa ttatttctag aggggaattg
      541 ttatccgctc acaattcccc tatagtgagt cgtattaatt tcgcgggatc gagatctcga
      601 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
      661 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
      721 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
      781 ccttgcatgc accattcctt gcg
//
