LOCUS       sequence-142727-         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CDC42EP1-V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE,
            Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
  TITLE     Identification of AMPK Phosphorylation Sites Reveals a Network of 
            Proteins Involved in Cell Invasion and Facilitates Large-Scale 
            Substrate Prediction.
  JOURNAL   Cell Metab. 2015 Nov 3;22(5):907-21. doi: 
            10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8.
  PUBMED    26456332
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.09.009"; journalName: "Cell Metab"; date: 
            "2015-11-3- 3"; volume: "22"; issue: "5"; pages: "907-21"
COMMENT     Sequence Label: pcDNA 3.2/V5-DEST CDC42EP1 WT
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    46..94
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     119..138
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(178..219)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    245..269
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gggtatcgac agagtgccag ccctgggacc gaaccccgcg tttatgaaca aacgacccaa
       61 cacccgtgcg ttttattctg tctttttatt gccgtcatag cgcgggttcc ttccggtatt
      121 gtctccttcc gtgtttcagt tagcctcccc cgtttaaact cattactaac cggtacgcgt
      181 agaatcgaga ccgaggagag ggttagggat aggcttacct tcgaaccgcg ggccctctag
      241 atcaaccact ttgtacaaga aagttgggca caccttgacc tcatcatcct cctcagcatc
      301 cgcaaattca aaggtgtttg cttgcactgt gctgggcact ggggtcctgc tgcctgcctg
      361 gggcgccctc cactcttcgt ccaggctctc ccaggaggcc cgggcttggg gcagcacctc
      421 cacccgctcc tgccgcgcat ccatctctgg gtagtggtgg ccgccatccc agcctgctcc
      481 ccagtgcctg ccgagggcag ggccagcagg tcctcgggga ccccctccca ctgggctgga
      541 cttcacctca gccactgggc ccaacccagc tgttacccca ttgggacagt gtccatgggg
      601 tgtggagctt gcggcagggg ctgggggatt tgcagcagga cccgtggggg ttgcggcagg
      661 tgctgggggg tttgcagtag tggctggggg gtttgcagca ggacccgtgg ggtttgcagt
      721 aggggctggg gggtttgcag cgggggctgg agtctcagct gcaggggctt ctggcaggct
      781 catgacccct agcagctcgc tgaggagtga gggcccaagg tcgaggtcca ggcggaagga
      841 caagagagag tcagagcggc gaagcccggg ctcagagggg aaggaaccat cccggtctcg
      901 gtcatgcggc ttttccgagc ggggcaggcg ggagatggtg ca
//
