LOCUS       sequence-142729-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a cmlc2::mCherry (red heart) reporter gene was inserted at the 3'end
            of pKHR4 mcs, that resides within donor fragments produced by I-SceI
            digestion. Genbank accession number is KU144824..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Hoshijima K, Jurynec MJ, Grunwald DJ
  TITLE     Precise Editing of the Zebrafish Genome Made Simple and Efficient.
  JOURNAL   Dev Cell. 2016 Mar 21;36(6):654-67. doi: 
            10.1016/j.devcel.2016.02.015.
  PUBMED    27003937
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2016.02"; journalName: "Dev Cell"; date: 
            "2016-03-21- 21"; volume: "36"; issue: "6"; pages: "654-67"
COMMENT     Sequence Label: pKHR7
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     65..81
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     65..81
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(115..131)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(116..132)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 cgcggtggcg gccaggtcct ttagtcgggt attcgggtag ggataacagg gtaatggcgg
       61 ccgctctaga actagtggat cccccgggct gcaggaattc gatatcaagc ttatcgatac
      121 cgtcgacctc gagaaatcag ttgtgttaaa taagagacat tcaaaataaa tgtaaatgag
      181 ctctccaaat cagcagactt aacattcttt aaaatgattg attcaatagt gataaaaatc
      241 aggcatagcc agttgtaact ttagataaat tacagaaaat gtcaaataca gagaaccgat
      301 tcttttttat gatacatcca agcacacatt taacacaatc caggcaaacc ccgaatttca
      361 cagtcacaag cactgtttgt acaagagctt tgcctaagga cacacagtct ctataagtcc
      421 aggtcgttgg tttcactctt attttaaaca tgtgacattt ttcctgccat cctgtcttag
      481 gctgctgttt gcttcattcc atgtcacatt aaattcctca gtagcacctt ttacacacac
      541 agccaatctt ttccagaaaa ttcaattgct ttgaagagat aatgtgtgaa caaatccatt
      601 tagaaaagga aaattaagaa tttgtaaaat catctgtaaa ttgttggcat tcttctgtat
      661 atgaacatca catcatttac aggtaaaggt ctggtcatta attatatgac aatttactgg
      721 tattattttg tgaaaggggc tattttcaat gcgttcatcc atccttttca tccctcaaat
      781 ctctcattca cgtccccctc cccatctgca cactttatct cattttccac cctgctggaa
      841 tctgagcact tgtgcagtta tcagggctcc tgtatttagg aggctctggg tgtccatgta
      901 ggggacgaac agaaacactg cagaccttta tagaagaaca attgataa
//
