LOCUS       sequence-142730-        2839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tetA landing pad template plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Tas H, Nguyen CT, Patel R, Kim NH, Kuhlman TE
  TITLE     An Integrated System for Precise Genome Modification in Escherichia 
            coli.
  JOURNAL   PLoS One. 2015 Sep 2;10(9):e0136963. doi: 
            10.1371/journal.pone.0136963. eCollection 2015.
  PUBMED    26332675
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-2"; volume: "10(9):e0136963. doi"; pages: " 
            10.1371/journal.pone.0136963. eCollection 2015"
COMMENT     Sequence Label: pTKLP-tetA
FEATURES             Location/Qualifiers
     source          1..2839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             120..1325
                     /codon_start=1
                     /gene="tetA from transposon Tn10"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFGV
                     LLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRLLS
                     GITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSPFFI
                     AALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYFSAQL
                     IGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKTAVLLG
                     FIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGALQGLLVS
                     LTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQAQGSKQET
                     SA"
     rep_origin      1388..1744
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(join(1841..2632,2633..2701))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2464..2483
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2702..2806)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tacggcccca aggtccaaac ggtgataggg ataacagggt aatgtaccat ttacgttgac
       61 accacctttc gcgtatggca tgacagcgcc cggaagagag tcaattcagg gtggtgaata
      121 tgaatagttc gacaaagatc gcattggtaa ttacgttgct cgatgccatg gggattggcc
      181 ttatcatgcc agtcttgcca acgttattac gtgaatttat tgcttcggaa gatatcgcta
      241 accactttgg cgtattgctt gcactttatg cgttaatgca ggttatcttt gctccttggc
      301 ttggaaaaat gtctgaccga tttggtcggc gcccagtgct gttgttgtca ttaataggcg
      361 catcgctgga ttacttattg ctggcttttt caagtgcgct ttggatgctg tatttaggcc
      421 gtttgctttc agggatcaca ggagctactg gggctgtcgc ggcatcggtc attgccgata
      481 ccacctcagc ttctcaacgc gtgaagtggt tcggttggtt aggggcaagt tttgggcttg
      541 gtttaatagc ggggcctatt attggtggtt ttgcaggaga gatttcaccg catagtccct
      601 tttttatcgc tgcgttgcta aatattgtca ctttccttgt ggttatgttt tggttccgtg
      661 aaaccaaaaa tacacgtgat aatacagata ccgaagtagg ggttgagacg caatcaaatt
      721 cggtgtacat cactttattt aaaacgatgc ccattttgtt gattatttat ttttcagcgc
      781 aattgatagg ccaaattccc gcaacggtgt gggtgctatt taccgaaaat cgttttggat
      841 ggaatagcat gatggttggc ttttcattag cgggtcttgg tcttttacac tcagtattcc
      901 aagcctttgt ggcaggaaga atagccacta aatggggcga aaaaacggca gtactgctcg
      961 gatttattgc agatagtagt gcatttgcct ttttagcgtt tatatctgaa ggttggttag
     1021 ttttccctgt tttaatttta ttggctggtg gtgggatcgc tttacctgca ttacagggag
     1081 tgatgtctat ccaaacaaag agtcatcagc aaggtgcttt acagggatta ttggtgagcc
     1141 ttaccaatgc aaccggtgtt attggcccat tactgtttgc tgttatttat aatcattcac
     1201 taccaatttg ggatggctgg atttggatta ttggtttagc gttttactgt attattatcc
     1261 tgctatcaat gaccttcatg ttgacccctc aagctcaggg gagtaaacag gagacaagtg
     1321 cttagtaggg ataacagggt aatgatggcg cctcatccct gaagccaagg accatggcta
     1381 attcccatgt cagccgttaa gtgttcctgt gtcactcaaa attgctttga gaggctctaa
     1441 gggcttctca gtgcgttaca tccctggctt gttgtccaca accgttaaac cttaaaagct
     1501 ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga ggctatttaa
     1561 gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt gaaacatgag
     1621 agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta gttcgttaaa
     1681 catgagagct tagtacgtta aacatgagag cttagtacgt gaaacatgag agcttagtac
     1741 gtactatcaa caggttgaac tgcggatctt gcggccgcaa aaattaaaaa tgaagtttta
     1801 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     1861 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1921 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1981 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     2041 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     2101 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2161 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2221 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     2281 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     2341 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     2401 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     2461 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     2521 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     2581 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     2641 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     2701 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     2761 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     2821 aagtgccacc tgcatcgat
//
