LOCUS       sequence-142731-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a cmlc2::mCherry (red heart) reporter gene was inserted at the 3'end
            of pKHR4 mcs, that resides within donor fragments produced by I-SceI
            digestion. Genbank accession number is KU144824..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Hoshijima K, Jurynec MJ, Grunwald DJ
  TITLE     Precise Editing of the Zebrafish Genome Made Simple and Efficient.
  JOURNAL   Dev Cell. 2016 Mar 21;36(6):654-67. doi: 
            10.1016/j.devcel.2016.02.015.
  PUBMED    27003937
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2016.02"; journalName: "Dev Cell"; date: 
            "2016-03-21- 21"; volume: "36"; issue: "6"; pages: "654-67"
COMMENT     Sequence Label: pKHR7
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    114..248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(151..170)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     205..224
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(366..389)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(564..583)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cggtccttta gtcgggtatt cgggtaggga taacagggta atggcggctc gaaaaaaacc
       61 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
      121 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
      181 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
      241 tctggatcta cgtaatacga ctcactatag ttctagaggc gcgccttact tgtacagctc
      301 gtccatgccg ccggtggagt ggcggccctc ggcgcgctcg tactgttcca cgatggtgta
      361 gtcctcgttg tgggaggtga tgtccagctt gatgttgacg ttgtaggcgc cgggcagctg
      421 cacgggcttc ttggccttgt aggtggtctt gacctcggcg tcgtagtggc cgccgtcctt
      481 cagcttcagc ctctgcttga tctcgccctt cagggcgccg tcctcggggt acatccgctc
      541 ggaggaggcc tcccagccca tggtcttctt ctgcattacg gggccgtcgg aggggaagtt
      601 ggtgccgcgc agcttcacct tgtagatgaa ctcgccgtcc tgcagggagg agtcctgggt
      661 cacggtcacc acgccgccgt cctcgaagtt catcacgcgc tcccacttga agccctcggg
      721 gaaggacagc ttcaagtagt cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc
      781 gtacatgaac tgaggggaca ggatgtccca ggcgaagggc agggg
//
