LOCUS       sequence-142739-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CDC42EP1-V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE,
            Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
  TITLE     Identification of AMPK Phosphorylation Sites Reveals a Network of 
            Proteins Involved in Cell Invasion and Facilitates Large-Scale 
            Substrate Prediction.
  JOURNAL   Cell Metab. 2015 Nov 3;22(5):907-21. doi: 
            10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8.
  PUBMED    26456332
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.09.009"; journalName: "Cell Metab"; date: 
            "2015-11-3- 3"; volume: "22"; issue: "5"; pages: "907-21"
COMMENT     Sequence Label: pcDNA 3.2/V5-DEST CDC42EP1 S192A
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    100..124
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagtt aagctatcaa caagtttgta caaaaaagtt
      121 ggcatgcccg gcccccaggg gggcagaggc gccgccacca tgagcctggg caagctctcg
      181 cctgtgggct gggtgtccag ttcacaggga aagaggcggc tgactgcaga catgatcagc
      241 cacccactcg gggacttccg ccacaccatg catgtgggcc gtggcgggga tgtcttcggg
      301 gacacgtcct tcctcagcaa ccacggtggc agctccggga gcacccatcg ctcaccccgc
      361 agcttcctgg ccaagaagct gcagctggtg cggagggtgg gggcgccccc ccggaggatg
      421 gcatctcccc ctgcaccctc cccggctcca ccggccatct cccccatcat caagaacgcc
      481 atctccctgc cccagctcaa ccaggccgcc tacgacagcc tcgtggttgg caagctcagc
      541 ttcgacagca gccccaccag ctccacggac ggccactcca gctacggcct ggactctggg
      601 ttctgcacca tctcccgcct gccccgctcg gaaaagccgc atgaccgaga ccgggatggt
      661 tccttcccct ctgagcccgg gcttcgccgc tctgacgctc tcttgtcctt ccgcctggac
      721 ctcgaccttg ggccctcact cctcagcgag ctgctagggg tcatgagcct gccagaagcc
      781 cctgcagctg agactccagc ccccgctgca aaccccccag cccctactgc aaaccccacg
      841 ggtcctg
//
