LOCUS       sequence-142740-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CDC42EP1-V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE,
            Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
  TITLE     Identification of AMPK Phosphorylation Sites Reveals a Network of 
            Proteins Involved in Cell Invasion and Facilitates Large-Scale 
            Substrate Prediction.
  JOURNAL   Cell Metab. 2015 Nov 3;22(5):907-21. doi: 
            10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8.
  PUBMED    26456332
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.09.009"; journalName: "Cell Metab"; date: 
            "2015-11-3- 3"; volume: "22"; issue: "5"; pages: "907-21"
COMMENT     Sequence Label: pcDNA 3.2/V5-DEST CDC42EP1 S192A
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    45..93
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     118..137
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(177..218)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    244..268
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtatcgaca gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa acgacccaac
       61 acccgtgcgt tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg
      121 tctccttccg tgtttcagtt agcctccccc gtttaaactc attactaacc ggtacgcgta
      181 gaatcgagac cgaggagagg gttagggata ggcttacctt cgaaccgcgg gccctctaga
      241 tcaaccactt tgtacaagaa agttgggcac accttgacct catcatcctc ctcagcatcc
      301 gcaaattcaa aggtgtttgc ttgcactgtg ctgggcactg gggtcctgct gcctgcctgg
      361 ggcgccctcc actcttcgtc caggctctcc caggaggccc gggcttgggg cagcacctcc
      421 acccgctcct gccgcgcatc catctctggg tagtggtggc cgccatccca gcctgctccc
      481 cagtgcctgc cgagggcagg gccagcaggt cctcggggac cccctcccac tgggctggac
      541 ttcacctcag ccactgggcc caacccagct gttaccccat tgggacagtg tccatggggt
      601 gtggagcttg cggcaggggc tgggggattt gcagcaggac ccgtgggggt tgcggcaggt
      661 gctggggggt ttgcagtagt ggctgggggg tttgcagcag gacccgtggg gtttgcagta
      721 ggggctgggg ggtttgcagc gggggctgga gtctcagctg caggggcttc tggcaggctc
      781 atgaccccta gcagctcgct gaggagtgag ggcccaaggt cgaggtccag gcggaaggac
      841 aagagag
//
