LOCUS       sequence-142741-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Output plasmid: PBM3R1-YFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Nielsen AA, Der BS, Shin J, Vaidyanathan P, Paralanov V, Strychalski
            EA, Ross D, Densmore D, Voigt CA
  TITLE     Genetic circuit design automation.
  JOURNAL   Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
  PUBMED    27034378
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-04-1"; volume: "352(6281):aac7341. doi"; pages: " 
            10.1126/science.aac7341"
COMMENT     Sequence Label: pAN4023
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(45..758,759..761,762..764))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKLHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(90..111)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     439..458
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     698..719
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ggcccccctt tcgggaggcc tcttttctgg aatttggtac cgagttactt gtacagctcg
       61 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
      121 ttctcgttgg ggtctttgct cagggcggac tggtagctaa ggtagtggtt gtcgggcagc
      181 agcacggggc cgtcgccgat tggggtgttc tgctggtagt ggtcggcgag ctgcacgctg
      241 ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg
      301 gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg
      361 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
      421 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
      481 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gcagcttcat gtggtcgggg
      541 tagcgggcga agcattgcag gccgtagccg aaggtggtca cgagggtggg ccagggcacg
      601 ggcagcttgc ctgtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
      661 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
      721 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatctagta tttccc
//
