LOCUS       sequence-142746-        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a plasmid derived from pKHR5: in addition to Venus reporter gene, it
            also contains cmlc2::mCherry (red heart) reporter gene at 3' end of 
            the mcs as pKHR7. Genbank accession number is KU144825..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Hoshijima K, Jurynec MJ, Grunwald DJ
  TITLE     Precise Editing of the Zebrafish Genome Made Simple and Efficient.
  JOURNAL   Dev Cell. 2016 Mar 21;36(6):654-67. doi: 
            10.1016/j.devcel.2016.02.015.
  PUBMED    27003937
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2016.02"; journalName: "Dev Cell"; date: 
            "2016-03-21- 21"; volume: "36"; issue: "6"; pages: "654-67"
COMMENT     Sequence Label: pKHR8
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     19..38
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     278..299
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    910..943
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(945..978)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(993..1009)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(994..1010)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
       61 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
      121 tagcgggcga agcactgcag gccgtagccc agggtggtca cgagggtggg ccagggcacg
      181 ggcagcttgc cggtggtgca gatcagcttc agggtcagct tgccgtaggt ggcatcgccc
      241 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
      301 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatgttgtc agacctggta
      361 actccttact gtaacttatt gcacctgatg aacaaaagat gtgaactgaa cgcaagtagg
      421 aggctaatag aggctagtac agtacaaaga tccccaaatg atgtgtgccg gccttagtgg
      481 aggcagaccc ctggttatat acctgtgctg cagaaagagg ggcttacaaa tggatctctc
      541 tggaatggca ttatctcatg gtgggaacag tgttgtcagc agaatcagcg gggtggactc
      601 tgacctgaga atgacagtct agcactgaat ggctcagaca atactcttgt ggccaactat
      661 caccggaaaa taagtgcctg ccaaaacaaa ccagaccaga tgaagctaca tggctgtaga
      721 acgtcagtat ttaatgtgtt gtggccactt ggttcaccag atttaagagc taattttttt
      781 aaatatcaca gataatcaat cgcttttttt ttttcttgac tgaaaagctt agactggaaa
      841 attctttgaa aaagtttgca ctacagaaaa taaatacata acatcctgca ctgaatgcac
      901 actattaatg aagttcctat tctctagaaa gtataggaac ttcaataact tcgtatagca
      961 tacattatac gaagttatga tatcaagctt atcgataccg tcgacctcga gaa
//
