LOCUS       sequence-142756-        5962 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase subunits and associated hypothetical protein with 
            N-terminal His6 tag (AaPurS, H6AaOrfY, AaPurQ, AaPurL-G209D).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK571
FEATURES             Location/Qualifiers
     source          1..5962
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             431..448
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3741..3757)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3741..3757)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3754..3776)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3765..3781
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3789..3795,3796..3813,3814..3819))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3834..3855
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3972..3989)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4143..4731)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4223..4242)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4902..5693,5694..5762))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5525..5544
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5763..5867)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5935..5953
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgaa ggtacgtgta accgtcatgc tgaaggacgg tgttctggac ccgcagggca
      241 aggccatcag ccacgcattg cagacacttg gttttgaagg cgtgcacgat gtgcgcgtgg
      301 gtaaggtgat tgaactggat ctgccgggtg ccaaagccga ggaagcccag aaaaaggccg
      361 atgccatggc gcgtgatctg ctagccaacc tggtgattga agattactct gtagaggtgg
      421 tggaatgagc caccaccacc accaccacaa gcgcaccctt cttatagcgc tggcattggc
      481 atgcgcggca ccgggcttgg cccatgccca gcgcattgcg cccatgaagg caggtaactt
      541 tgcccgcctt tgtacgcgcg ccactagtgc tggcatttgc gatgcctata ttggcggcct
      601 gacggatggg gtctctcttt ccaaaatcaa tgatcggaac gagggggatc cttctgcgcc
      661 tgctggtttt tgtgttcccg ctacagaaac catgcccgtt atgcgctcca aggtacttac
      721 ctggctgaaa gcgcattctg aaacactgga caagccggtt ggtgaaagtg tttttgccgc
      781 cctgcacgcc tcttatccgt gcagtgtgaa aaaatgaaag cagcaattat cgtattcccc
      841 ggtaccaacc gggagcgcga tatggccatt gccctcaagc gcgttacagg gcaggccccg
      901 cgcatgatct ggcaccatga aacagatctg ggagataccg atctagtcgt gctgccaggt
      961 ggtttcagct atggcgatta tctgcgctgt ggtgccatgg cagcgcattc tcccatcatg
     1021 gcggctatca aagcctttgc ccaaaagggt ggccatattt tgggtgtgtg caacggcttc
     1081 cagattttaa cggaaacagg gcttctgccc ggtgctctgt tgcgcaacgc caacctgcgc
     1141 tttctctctc aggattgcta cctgaaggtg gaagcagaga acacaccttt cactcacaag
     1201 tggaagaagg gtgatgtttt ccgcacccct ctggcccatg gtgatggcaa ttacacggca
     1261 gatatcgcca cgctggaacg gcttgaaggt gaaggtcttg tggctttcca ctatgccgat
     1321 gccaaaggca atgtgcaggc tgatgatgcc atcagcaacc ccaatggcag catcaacagc
     1381 attgcgggta ttctgagcga aaacaaacgc atttgcggca tgatgcctca tccagaagat
     1441 ctggttgatc ctctcatggg tggggaagat ggtctgccat tgttccaagg tctggtggag
     1501 gctgtagtcc ggtgaataag cctgtaaccg ttgatgaatc ccttgcccgt gaattcgggc
     1561 tgacatctga ggaatacggt cgtgtgctgt ccattatggg ccgcaccccc agcctgacag
     1621 agctgggcgt gttctctgtg atgtggtcgg aacactgctc ctacaaatct tcccgcaagt
     1681 ggctgcgcac gctgccaacc aaggcgccgt gggttattca tggccccggt gaaaatgctg
     1741 gtgtggtgga tattgggcag ggctatgccg ccatcttcaa gatggaaagc cataaccacc
     1801 catcctttat cgaaccctat cagggtgcgg ccacgggtgt ggggggcatt ctgcgcgatg
     1861 tgttcaccat gggcgcacgc cccattgcca acttgaacgc cctgcgtttt ggcagccccg
     1921 aaaaccccgt tacccgccgc gtgattgatg gcgtggtgcg tggtattggc ggctatggca
     1981 actgtgttgg cgtgcccacc gtaggcgggg aaatcaactt ccacccggcc tatgatggca
     2041 acccgctggt aaacgccatg acggttggtt tggcccgtaa ggaccgtatc ttcctttcgg
     2101 ctgcggcagg tgttggcaac ccggttgttt acgtggactc ccgcacaggg cgtgatggca
     2161 ttcatggtgc caccatgtct tccgccgagt ttgatgaaga tgccctttcc aagcgcccca
     2221 cggtacaggt gggtgacccc tttattgaaa agcttctgat tgaagcctgt ctggaactga
     2281 tggcaacaga tgccattatc gccattcagg acatgggcgc tgcgggtctg acatcttctt
     2341 ccgtggaaat ggcaggcaag ggcggtgtgg gcattgagct tgatctggat gccgtgcccc
     2401 agcgtgagcc gaacatgacg gcttatgaaa tgatgctgtc tgaaagccag gaacgcatgc
     2461 tgatggtgct gcgccctgac cggacagacg tggcccgcaa gatctttgaa aaatgggaac
     2521 tggattttgc cattattggg catctgacag ataccggcca catcgtcgtc aagcacaaag
     2581 gacagttgga agccgatatt ccgctggacc cattggcaga acaggcccct atttacgatc
     2641 ggcccgcaac accacccaag gcgccggctc cgctagaagg cgtgcatgca ccgctttctt
     2701 tggatgccac gttactgaag ctggttggtt cgccagatct ggcctcccgc gcatgggtat
     2761 ggaaccagta tgacagcact gtaggggggc agacagtccg ccgtccgggt gctgctgatg
     2821 ctgccattgt gaaggtggaa gatacagaca ttggtctggc catcaccaca gattgcaccc
     2881 cgcgttactg tcaggcagac cccaaaacag gtggcgcaca agctgttgca gaagcatggc
     2941 gtaacctaac agctactggc gcacgcccgc tggccgtaac ggataacctg aacttcgcca
     3001 acccggaaaa acccgaaatc atgggccagt ttgtggctgc ggttgaaggt atgggggaag
     3061 cctgtacagc gctggatttc ccggtcgtga gcggcaacgt gtctctttat aatgaaacac
     3121 gaaacccaga tggctccacc accgccattc tgcccacacc cgccattggc gccttgggcg
     3181 ttctggatga tgtgaacaag gctgttggcc ttggcatgca gccagaatct gacgtggtgc
     3241 tgattggtga aaccaagggt gagctcggcc agaccatctg gctgcgtgaa attctgggtc
     3301 aggaagctgg tgcaccgccg ccggtcgatc tggttgccga aaaacgcaat ggtgactttg
     3361 tacgcagcgc cattctggat ggcaccatta ccgcatgcca tgacattgct gacggtggca
     3421 ttatggttgc cgccgcagaa atggtgatgg caagtggcat tggatgtgag ctggaaagcc
     3481 cagattccgg tatccggcca gaagccttct ggtttggtga agatcaggcc cgctatctgg
     3541 cttgtgtagc aaacggcaaa gccctgtgcg aacgcgctca ggcagctggt gtgcctgcac
     3601 gcattattgc ccgctcaggc ggaaatgatt tgattctgcc cagcggcgtc accatatccg
     3661 cagcacgcct aaaaacggcc catgaagcat ttttcccgat gctgatgggc aactaactcg
     3721 agaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     3781 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     3841 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     3901 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     3961 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     4021 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     4081 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     4141 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     4201 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4261 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4321 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4381 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4441 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4501 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4561 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     4621 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4681 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4741 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4801 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4861 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     4921 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     4981 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     5041 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     5101 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     5161 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     5221 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     5281 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     5341 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     5401 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     5461 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     5521 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     5581 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     5641 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     5701 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     5761 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     5821 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     5881 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     5941 cgtatcacga ggccctttcg tc
//
