LOCUS       sequence-142774-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SEC14L3
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccat gagcggccga gttggagacc tgagccccaa
      121 acaggcagag accctggcca agttccgaga aaacgtccag gatgtgcttc ctgccctgcc
      181 caaccctgat gattatttcc ttctacgctg gctccgagct cggaattttg acttgcagaa
      241 gtcggaggct ttgctccgca agtacatgga gttccggaag accatggata ttgaccatat
      301 ccttgattgg cagcccccag aggtgatcca gaagtacatg cctgggggcc tgtgtggcta
      361 tgaccgtgat ggctgccccg tgtggtatga catcattggg ccacttgatc ccaaggggtt
      421 gctcttctca gtcaccaagc aggacctgct caagaccaag atgagggact gtgagcgcat
      481 cctgcatgag tgtgacctgc agacagagag gctagggaag aagattgaga ccatcgtgat
      541 gatatttgac tgtgagggcc tgggactgaa acacttctgg aaacctctgg tagaagtgta
      601 ccaggagttc tttggcctcc ttgaagagaa ttacccagag accctgaagt tcatgctcat
      661 cgtgaaagct accaaactgt tccctgtggg ctacaacctc atgaagccat tcctgagtga
      721 ggacactcgc aggaaaatta ttgtgttggg aa
//
