LOCUS       sequence-142782-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses the N-terminal fragment of manduca sexta soluble guanylyl 
            cyclase subunits alpha and beta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Hu X, Murata LB, Weichsel A, Brailey JL, Roberts SA, Nighorn A, 
            Montfort WR
  TITLE     Allostery in recombinant soluble guanylyl cyclase from Manduca 
            sexta.
  JOURNAL   J Biol Chem. 2008 Jul 25;283(30):20968-77. doi: 
            10.1074/jbc.M801501200. Epub 2008 May 30.
  PUBMED    18515359
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M801501200"; journalName: "J Biol Chem"; date: 
            "2008-07-25- 25"; volume: "283"; issue: "30"; pages: "20968-77"
COMMENT     Sequence Label: pETDuet-msGC-NT2
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    66..90
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             105..127
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             146..163
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg cgaaattaat acgactcact
       61 ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag
      121 aaggagatat accatgggca gcagccatca ccatcatcac cacagccagg atccgctcac
      181 tcttaagcat atgagtgagg ctttgcaact cctgactgct ccatcgaatg aatgcctcca
      241 tgcagctgtg acgtcactca cgaagaatca atcagatcac taccataaat acaattgttt
      301 gcgtagatta ccagacgacg taaaaacttg tagaaattac gcgtacctac aagaaattta
      361 cgacgccgtg cgagcaactg acagtgtcaa cacgaaggat tttatggcaa aattagggga
      421 atatttaatt ttaacagcct tctcgcacaa ttgtcggtta gagagagcgt ttaaatgttt
      481 aggcacaaat ttaacagaat ttctgaccac actagacagt gttcatgacg tgttgcacga
      541 tcaggacaca ccgcttaaag atgaaacgat ggagtacgaa gcgaatttcg tatgcacgac
      601 ctcgcaagaa gggaagatac agttacactt gaccacggaa agcgaaccag tggcgtacct
      661 tttagtaggc agtctgaaag ccatagcgaa acgactgtat gatacacaga cagacataag
      721 actgaggagc tataccaatg atcctaggag gtt
//
