LOCUS       sequence-142786-         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  based on pCX-C1 with F2A1/F2A2 inserted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  AUTHORS   De Giorgi M, Cinti A, Pelikant-Malecka I, Chisci E, Lavitrano M, 
            Giovannoni R, Smolenski RT
  TITLE     Co-expression of functional human Heme Oxygenase 1, 
            Ecto-5'-Nucleotidase and ecto-nucleoside triphosphate 
            diphosphohydrolase-1 by ""self-cleaving"" 2A peptide system.
  JOURNAL   Plasmid. 2015 May;79:22-9. doi: 10.1016/j.plasmid.2015.03.004. Epub 
            2015 Mar 14.
  PUBMED    25779031
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid."; 
            date: "2015-05"; volume: "79:22-9. doi"; pages: " 
            10.1016/j.plasmid.2015.03.004. Epub 2015 Mar 14"
COMMENT     Sequence Label: pCX-2A-2A
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(63..128)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             complement(156..221)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     primer_bind     complement(255..274)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(336..358)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ccagccacca ccttctgata ggcagcctgc acctgaggag tgaattcgct agcgccgctg
       61 ccgggccctg ggttggactc cacgtctccc gccaacttga gaaggtcaaa attcaaagtc
      121 tgtttcacgg taccgaattc gctagcgccg ctgccgggcc ctgggttgga ctccacgtct
      181 cccgccaact tgagaaggtc aaaattcaaa gtctgtttca cggtaccgaa ttctttgcca
      241 aaatgatgag acagcacaat aaccagcacg ttgcccagga gctgtaggaa aaagaagaag
      301 gcatgaacat ggttagcaga ggctctagag ccgccggtca cacgccagaa gccgaacccc
      361 gccctgcccc gtcccccccg aaggcagccg tccccccgcg gacagccccg aggctggaga
      421 gggagaaggg gacggcggcg cggcgacgca cgaaggccct ccccgcccat ttccttcctg
      481 ccggcgccgc accgcttcgc cccgcgcccg ctagaggggg tgcggcggcg cctcccagat
      541 ttcggctccg cacagatttg ggacaaagga agtccctgcg ccctctcgca cgattaccat
      601 aaaaggcaat ggctgcggct cgccgcgcct cgacagccgc cggcgctccg ggggccgccg
      661 cgcccctccc ccgag
//
