LOCUS       sequence-142787-         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  based on pCX-C1 with F2A1/F2A2 inserted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   De Giorgi M, Cinti A, Pelikant-Malecka I, Chisci E, Lavitrano M, 
            Giovannoni R, Smolenski RT
  TITLE     Co-expression of functional human Heme Oxygenase 1, 
            Ecto-5'-Nucleotidase and ecto-nucleoside triphosphate 
            diphosphohydrolase-1 by ""self-cleaving"" 2A peptide system.
  JOURNAL   Plasmid. 2015 May;79:22-9. doi: 10.1016/j.plasmid.2015.03.004. Epub 
            2015 Mar 14.
  PUBMED    25779031
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid."; 
            date: "2015-05"; volume: "79:22-9. doi"; pages: " 
            10.1016/j.plasmid.2015.03.004. Epub 2015 Mar 14"
COMMENT     Sequence Label: pCX-2A-2A
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..143
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     primer_bind     complement(242..261)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    307..362
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(361..380)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(723..739)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(723..739)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 attttgacct tctcaagttg gcgggagacg tggagtccaa cccagggccc ggcagcggcg
       61 ctagcgaatt cggtaccgtg aaacagactt tgaattttga ccttctcaag ttggcgggag
      121 acgtggagtc caacccaggg cccggcagcg gcgctagcga attcactcct caggtgcagg
      181 ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag
      241 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
      301 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
      361 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt
      421 agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag
      481 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga
      541 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa
      601 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca
      661 tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca
      721 tggtcatagc tgtttcctgt gtgaa
//
