LOCUS       sequence-142792-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of HO1 and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Cinti A, De Giorgi M, Chisci E, Arena C, Galimberti G, Farina L, 
            Bugarin C, Rivolta I, Gaipa G, Smolenski RT, Cerrito MG, Lavitrano 
            M, Giovannoni R
  TITLE     Simultaneous Overexpression of Functional Human HO-1, E5NT and 
            ENTPD1 Protects Murine Fibroblasts against TNF-alpha-Induced Injury 
            In Vitro.
  JOURNAL   PLoS One. 2015 Oct 29;10(10):e0141933. doi: 
            10.1371/journal.pone.0141933. eCollection 2015.
  PUBMED    26513260
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-10-29"; volume: "10(10):e0141933. doi"; pages: " 
            10.1371/journal.pone.0141933. eCollection 2015"
COMMENT     Sequence Label: pCX-HO1-2A-EGFP
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(61..774,775..777,778..780))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(64..774,775..777,778..780))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(106..127)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     455..474
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     714..735
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gccacaccag ccaccacctt ctgataggca gcctgcacct gaggagtgaa ttctgagtcc
       61 ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
      121 gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta
      181 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
      241 ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc
      301 gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt
      361 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
      421 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
      481 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
      541 cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag
      601 ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc
      661 gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc
      721 gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat
      781 cttaaggccg ctgccgggcc ctgggttgga ctccacgtct cccgccaact tgagaaggtc
      841 aaa
//
