LOCUS       sequence-142798-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Wolfson RL, Chantranupong L, Saxton RA, Shen K, Scaria SM, Cantor 
            JR, Sabatini DM
  TITLE     Sestrin2 is a leucine sensor for the mTORC1 pathway.
  JOURNAL   Science. 2016 Jan 1;351(6268):43-8. doi: 10.1126/science.aab2674. 
            Epub 2015 Oct 8.
  PUBMED    26449471
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aab2674"; journalName: "Science"; date: "2016-01-1-
            1"; volume: "351"; issue: "6268"; pages: "43-8"
COMMENT     Sequence Label: pS2-FLAG-Sestrin2-L261A
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      771..780
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             780..803
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tctataatta gcagcaccgg ataggacgcg agccaaacct ctcttaaccc tagccagtcc
       61 ctccaggaac tgaaaagatg aagaattcat ttaggagatt tcacactcgg ctgtttggcc
      121 tagcccagtg aagtcccttt tcatcacgca accctaatga ctcttagggc tgtcactgcc
      181 gtctggtgtt gccagggatc tgactaaacc gtcttgcaaa agtttgcagc attcacccga
      241 ggcggactac aactcccaga attccgggcg ctccccggtg ctgattggcg gagcccgtga
      301 ctccaggggg cggagtttcc cccgcctccc cggggaaaca gacctctgat tggctggacc
      361 gccgggcttt tgtgtccccg ccctcccccg gggaccgcag cgggctgaac tgctggtgtc
      421 agagcccggc gagcgctggc agttccgcgg cggggatgct gaggagcgct gggtccggga
      481 gcagccctgg cccctgcgga cttccgaggc cgtgaaaacc cctgcgctgc ggcccttccc
      541 aggcccccga ggccgttcgc cgttcccgaa gcccgactgg gggaagagtc cagcaccaaa
      601 gcggccgttc tcggattccg gagcgttctg gagccccgag agacgccccg gggttctaga
      661 agctccccgg cggcgcccag tcccggcttc attcgggcgt ccctccgaaa cccactcggg
      721 tgcacgggtc gtcggcgagc cgcgaccggg tcctggcgcg caccaccggt gccaccatgg
      781 actacaagga tgacgatgac aagggtggtg gtggtggtgc ctcacaatga tcgtggcgga
      841 ctccgagtgc cgcgcagagc tcaaggacta cctgcggttc gccccgggcg gcgtcggcga
      901 ctcgggcccc ggaagagaa
//
