LOCUS       sequence-14280-e        1334 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Ryffel GU, Werdien D, Turan G, Gerhards A, Goosses S, Senkel S
  TITLE     Tagging muscle cell lineages in development and tail regeneration 
            using Cre recombinase in transgenic Xenopus.
  JOURNAL   Nucleic Acids Res. 2003 Apr 15. 31(8):e44.
  PUBMED    12682379
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2003 Apr 15. 31(8):e44."
COMMENT     Sequence Label: pCARCre
FEATURES             Location/Qualifiers
     source          1..1334
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..26
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8..25
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             63..1091
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     polyA_signal    1126..1260
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1150..1169)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1204..1223
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 aagcttgatt taggtgacac tatagaatac aagctacttg ttctttttgc aggatctccg
       61 atatgtccaa tttactgacc gtacaccaaa atttgcctgc attaccggtc gatgcaacga
      121 gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg ttttctgagc
      181 atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc aagttgaata
      241 accggaaatg gtttcccgca gaacctgaag atgttcgcga ttatcttcta tatcttcagg
      301 cgcgcggtct ggcagtaaaa actatccagc aacatttggg ccagctaaac atgcttcatc
      361 gtcggtccgg gctgccacga ccaagtgaca gcaatgctgt ttcactggtt atgcggcgga
      421 tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca ggctctagcg ttcgaacgca
      481 ctgatttcga ccaggttcgt tcactcatgg aaaatagcga tcgctgccag gatatacgta
      541 atctggcatt tctggggatt gcttataaca ccctgttacg tatagccgaa attgccagga
      601 tcagggttaa agatatctca cgtactgacg gtgggagaat gttaatccat attggcagaa
      661 cgaaaacgct ggttagcacc gcaggtgtag agaaggcact tagcctgggg gtaactaaac
      721 tggtcgagcg atggatttcc gtctctggtg tagctgatga tccgaataac tacctgtttt
      781 gccgggtcag aaaaaatggt gttgccgcgc catctgccac cagccagcta tcaactcgcg
      841 ccctggaagg gatttttgaa gcaactcatc gattgattta cggcgctaag gatgactctg
      901 gtcagagata cctggcctgg tctggacaca gtgcccgtgt cggagccgcg cgagatatgg
      961 cccgcgctgg agtttcaata ccggagatca tgcaagctgg tggctggacc aatgtaaata
     1021 ttgtcatgaa ctatatccgt aacctggata gtgaaacagg ggcaatggtg cgcctgctgg
     1081 aagatggcga tggttctcta gaactatagt gagtcgtatt acgtagatcc agacatgata
     1141 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     1201 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     1261 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     1321 taattcgcgg ccgc
//
