LOCUS       sequence-142801-        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MTFR1L-V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE,
            Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
  TITLE     Identification of AMPK Phosphorylation Sites Reveals a Network of 
            Proteins Involved in Cell Invasion and Facilitates Large-Scale 
            Substrate Prediction.
  JOURNAL   Cell Metab. 2015 Nov 3;22(5):907-21. doi: 
            10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8.
  PUBMED    26456332
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.09.009"; journalName: "Cell Metab"; date: 
            "2015-11-3- 3"; volume: "22"; issue: "5"; pages: "907-21"
COMMENT     Sequence Label: pcDNA 3.2/V5-DEST MTFR1L-V5
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    50..98
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     123..142
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(182..223)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    249..273
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac
       61 ccaacacccg tgcgttttat tctgtctttt tattgccgtc atagcgcggg ttccttccgg
      121 tattgtctcc ttccgtgttt cagttagcct cccccgttta aactcattac taaccggtac
      181 gcgtagaatc gagaccgagg agagggttag ggataggctt accttcgaac cgcgggccct
      241 ctagatcaac cactttgtac aagaaagttg ggcaatttcc ttttctactg atatcttctt
      301 cctttagagc aaacttcctc cttaggacct cagagataag cacagcaggg tcttcctcct
      361 tcaataaact ccggttcaga tcttgactct gtttcattcg gtgaaagtcc ttcaggatac
      421 ccatcatgtc tgcaaagctg ctggccttgg acaaagagac gcagtcatcc tcttcagacg
      481 aactgcaggc agctttgtgt tctggtttgc aacattcagg gctggcagaa caaagcaggg
      541 ggactgatgg aagctcaggg acctccacct ctgtctcctc ggtgatgtca ctaatgacaa
      601 aggaagttgt agagtggaat gaggatccaa aacaaggtaa gggagaagag acatttgaac
      661 ttcctggaga taagaaatct ggcgttaatg aagccgaagc tgcatcagct gccactatct
      721 ttgcaatctg gctgcgcaag tggctcagct cgtcctgcag ctcagtagtg cggcttaggg
      781 ctggcagagc tggcttcttc agggccagaa gcctctcctc ggacccacgc aggttgggaa
      841 cagaggcatt gcgctgcatg acaatcagag ggctgggttt ccaggtgtgc ctcagggggc
      901 gcgtatcact cctgacccgg gcatatgtct cctcttcatc cgcagcaatc caggcgatgt
      961 cagccagggt agggactggg ggcacatctc tcaaacacag gtca
//
