LOCUS       sequence-142809-         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RBM14-V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE,
            Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
  TITLE     Identification of AMPK Phosphorylation Sites Reveals a Network of 
            Proteins Involved in Cell Invasion and Facilitates Large-Scale 
            Substrate Prediction.
  JOURNAL   Cell Metab. 2015 Nov 3;22(5):907-21. doi: 
            10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8.
  PUBMED    26456332
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.09.009"; journalName: "Cell Metab"; date: 
            "2015-11-3- 3"; volume: "22"; issue: "5"; pages: "907-21"
COMMENT     Sequence Label: pcDNA 3.2/V5-DEST RBM14-V5
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    53..101
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     126..145
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(185..226)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    252..276
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac
       61 gacccaacac ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc
      121 cggtattgtc tccttccgtg tttcagttag cctcccccgt ttaaactcat tactaaccgg
      181 tacgcgtaga atcgagaccg aggagagggt tagggatagg cttaccttcg aaccgcgggc
      241 cctctagatc aaccactttg tacaagaaag ttgggtacat gcggcgctgg tagccagagt
      301 gcatctgagc cgcccgcagg taatcattat aggagcccga atagcgtgcg taatcggaat
      361 gggcatcggg caggcgacgg taatccagcg aggactttgt cggcgagcgg cggaacgaaa
      421 gctgcgactc tgataaacgg cggtaatcag agagctcggc taaacgccgg tcggaaccat
      481 accttttcga catggcgaca gcctttttgt agggatcgtc gtagctggcc cggggtgggg
      541 agaggcgggt acgctcatag ggcggcgggg tgctgttggc gttggcaacg gccccctggg
      601 acatggacag gtaggctgca gacggctgac ctgccccatc gtaggcattg cctggctggc
      661 cgcggtagga ggcagcagcc tggggatgct gctgggcagc ataggaagca gccaatgagg
      721 ctgatgactg agtgcggtaa ggagctgcca gggtggcaga aggt
//
