LOCUS       sequence-142813-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible
            manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE,
            Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
  TITLE     Identification of AMPK Phosphorylation Sites Reveals a Network of 
            Proteins Involved in Cell Invasion and Facilitates Large-Scale 
            Substrate Prediction.
  JOURNAL   Cell Metab. 2015 Nov 3;22(5):907-21. doi: 
            10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8.
  PUBMED    26456332
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.09.009"; journalName: "Cell Metab"; date: 
            "2015-11-3- 3"; volume: "22"; issue: "5"; pages: "907-21"
COMMENT     Sequence Label: FUW TetO HA-AMPKa1 WT
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..47
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             135..161
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
       61 atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc cgcggccccg
      121 aattcgaaac catgtatgat gttcctgatt atgctagcct cccggggaat tcggcgacag
      181 ccgagaagca gaaacacgac gggcgggtga agatcggcca ctacattctg ggtgacacgc
      241 tgggggtcgg caccttcggc aaagtgaagg ttggcaaaca tgaattgact gggcataaag
      301 tagctgtgaa gatactcaat cgacagaaga ttcggagcct tgatgtggta ggaaaaatcc
      361 gcagagaaat tcagaacctc aagcttttca ggcatcctca tataattaaa ctgtaccagg
      421 tcatcagtac accatctgat attttcatgg tgatggaata tgtctcagga ggagagctat
      481 ttgattatat ctgtaagaat ggaaggctgg atgaaaaaga aagtcggcgt ctgttccaac
      541 agatcctttc tggtgtggat tattgtcaca ggcatatggt ggtccataga gatttgaaac
      601 ctgaaaatgt cctgcttgat gcacacatga atgcaaagat agctgatttt ggtctttcaa
      661 acatgatgtc agatggtgaa tttttaagaa caagttgtgg ctcacccaac tatgctgcac
      721 cagaagtaat ttcaggaaga ttgtatgcag gcccagaggt agatatatgg agcagtgggg
      781 ttattctcta tgctttatta tgtggaaccc ttccatttga tgatgaccat gtgccaactc
      841 tttttaagaa gatatgtgat gggatcttct atacccctca atatttaaat ccttctgtga
      901 ttagcctttt gaaacatatg ctgcaggtgg atcccatgaa gagggccaca
//
