LOCUS       sequence-142814-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of mCherry-NBR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Kenific CM, Stehbens SJ, Goldsmith J, Leidal AM, Faure N, Ye J, 
            Wittmann T, Debnath J
  TITLE     NBR1 enables autophagy-dependent focal adhesion turnover.
  JOURNAL   J Cell Biol. 2016 Feb 29;212(5):577-590. Epub 2016 Feb 22.
  PUBMED    26903539
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2016-02-29"; volume: "212(5)"; pages: "577-590. Epub 
            2016 Feb 22"
COMMENT     Sequence Label: pMXspuro-mCherry-NBR1
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..753
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(191..209)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     459..478
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 catcctctag actgccggat ctagctagtt aattaaggat ctaccatggt gagcaagggc
       61 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      121 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      181 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      241 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      301 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      361 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      421 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
      481 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
      541 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
      601 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
      661 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
      721 cactccaccg gcggcatgga cgagctgtac aagtccggac tcagatccgc tgg
//
