LOCUS       sequence-142823-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible
            manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE,
            Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
  TITLE     Identification of AMPK Phosphorylation Sites Reveals a Network of 
            Proteins Involved in Cell Invasion and Facilitates Large-Scale 
            Substrate Prediction.
  JOURNAL   Cell Metab. 2015 Nov 3;22(5):907-21. doi: 
            10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8.
  PUBMED    26456332
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.09.009"; journalName: "Cell Metab"; date: 
            "2015-11-3- 3"; volume: "22"; issue: "5"; pages: "907-21"
COMMENT     Sequence Label: FUW TetO HA-AMPKa1 AS
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     19..43
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             131..157
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
       61 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt
      121 cgaaaccatg tatgatgttc ctgattatgc tagcctcccg gggaattcgg cgacagccga
      181 gaagcagaaa cacgacgggc gggtgaagat cggccactac attctgggtg acacgctggg
      241 ggtcggcacc ttcggcaaag tgaaggttgg caaacatgaa ttgactgggc ataaagtagc
      301 tgtgaagata ctcaatcgac agaagattcg gagccttgat gtggtaggaa aaatccgcag
      361 agaaattcag aacctcaagc ttttcaggca tcctcatata attaaactgt accaggtcat
      421 cagtacacca tctgatattt tcatggtggg ggaatatgtc tcaggaggag agctatttga
      481 ttatatctgt aagaatggaa ggctggatga aaaagaaagt cggcgtctgt tccaacagat
      541 cctttctggt gtggattatt gtcacaggca tatggtggtc catagagatt tgaaacctga
      601 aaatgtcctg cttgatgcac acatgaatgc aaagatagct gattttggtc tttcaaacat
      661 gatgtcagat ggtgaatttt taagaacaag ttgtggctca cccaactatg ctgcaccaga
      721 agtaatttca ggaagattgt atgcaggccc agaggtagat atatggagca gtggggttat
      781 tctctatgct ttattatgtg gaacccttcc atttgatgat gaccatgtgc caactctttt
      841 taagaagata tgtgatggga tcttctatac ccctcaatat tt
//
