LOCUS       sequence-142830-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible
            manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE,
            Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
  TITLE     Identification of AMPK Phosphorylation Sites Reveals a Network of 
            Proteins Involved in Cell Invasion and Facilitates Large-Scale 
            Substrate Prediction.
  JOURNAL   Cell Metab. 2015 Nov 3;22(5):907-21. doi: 
            10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8.
  PUBMED    26456332
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.09.009"; journalName: "Cell Metab"; date: 
            "2015-11-3- 3"; volume: "22"; issue: "5"; pages: "907-21"
COMMENT     Sequence Label: FUW TetO HA-AMPKa2 AS
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     19..43
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             131..157
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
       61 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt
      121 cgaaaccatg tatgatgttc ctgattatgc tagcctcccg gggaattcgg ctgagaagca
      181 gaagcacgac gggcgggtga agatcggaca ctacgtgctg ggcgacacgc tgggcgtcgg
      241 caccttcggc aaagtgaaga ttggagaaca tcaattaaca ggccataaag tggcagttaa
      301 aatcttaaat agacagaaga ttcgcagttt agatgttgtt ggaaaaataa aacgagaaat
      361 tcaaaatcta aaactctttc gtcatcctca tattatcaaa ctataccagg tgatcagcac
      421 tccaacagat ttttttatgg taggggaata tgtgtctgga ggtgaattat ttgactacat
      481 ctgtaagcat ggacgggttg aagagatgga agccaggcgg ctctttcagc agattctgtc
      541 tgctgtggat tactgtcata ggcatatggt tgttcatcga gacctgaaac cagagaatgt
      601 cctgttggat gcacacatga atgccaagat agccgatttc ggattatcta atatgatgtc
      661 agatggtgaa tttctgagaa ctagttgcgg atctccaaat tatgcagcac ctgaagtcat
      721 ctcaggcaga ttgtatgcag gtcctgaagt tgatatctgg agctgtggtg ttatcttgta
      781 tgctcttctt tgtggcaccc tcccatttga tgatgagcat gtacctacgt tatttaagaa
      841 gatccgaggg ggtgtctttt atatcccaga atatctcaat cgttctgtcg ccactctcct
      901 gatgcatatg ctgcaggttg acccactgaa acgagcaact atcaaag
//
