LOCUS       sequence-142848-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses NET1A-V5 in mammalian cells in a doxycycline inducible 
            manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE,
            Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
  TITLE     Identification of AMPK Phosphorylation Sites Reveals a Network of 
            Proteins Involved in Cell Invasion and Facilitates Large-Scale 
            Substrate Prediction.
  JOURNAL   Cell Metab. 2015 Nov 3;22(5):907-21. doi: 
            10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8.
  PUBMED    26456332
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.09.009"; journalName: "Cell Metab"; date: 
            "2015-11-3- 3"; volume: "22"; issue: "5"; pages: "907-21"
COMMENT     Sequence Label: FUW TetO NET1A-V5 S46A
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     19..43
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      159..168
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
       61 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt
      121 cgaagactag gatcctctag agaagactat taattaaacc atggtggcac atgatgagac
      181 tggaggtctc ctacctatta aaaggaccat acgagtccta gatgtcaata accagtcctt
      241 cagagaacaa gaggagccaa gcaataaaag agttcgacct ctggctcgtg tcacggcctt
      301 ggcaaattta atctctcctg taagaaatgg agctgtcaga cgttttggtc aaacaataca
      361 gtcatttacc cttcgtggtg accacagatc cccagcctct gcccagaagt tttctagcag
      421 gtcaacagtc ccaacacccg ccaagagaag gagcagtgca ctgtggtcag agatgctgga
      481 catcaccatg aaggagtctc tcaccaccag ggagatcaga cggcaggagg caatatatga
      541 aatgtcccga ggtgaacagg atttaattga ggatctcaaa cttgcaagaa aggcctacca
      601 tgaccccatg ttaaagttgt ccatcatgtc agaagaggaa ctcacacata tatttggtga
      661 tctggactct tacatacctc tgcatgaaga tttgttgaca agaataggag aagcaaccaa
      721 gcctgatgga acagtggagc agattggtca cattctcgtg agctggttac cgcgcttgaa
      781 tgcctacaga ggttactgta gtaaccagct ggcagccaaa gctcttcttg atcaaaagaa
      841 acaggatcca agagtccaag acttcctcca gcgatgtctc gagtctccct tcagtcgaaa
      901 actagatctt tggagtttc
//
