LOCUS       sequence-142849-        7824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SLC24A3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Kraev A, Quednau BD, Leach S, Li XF, Dong H, Winkfein R, Perizzolo 
            M, Cai X, Yang R, Philipson KD, Lytton J
  TITLE     Molecular cloning of a third member of the potassium-dependent 
            sodium-calcium exchanger gene family, NCKX3.
  JOURNAL   J Biol Chem. 2001 Jun 22;276(25):23161-72. Epub 2001 Apr 9.
  PUBMED    11294880
REFERENCE   2  (bases 1 to 7824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2001-06-22"; volume: "276(25)"; pages: "23161-72. 
            Epub 2001 Apr 9"
COMMENT     Sequence Label: Mouse NCKX3 (pcDNA3.1+)
FEATURES             Location/Qualifiers
     source          1..7824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3414..3431)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3420..3644
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3690..4118
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3777..3796)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3987..4008
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4127..4147)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4132..4462
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4313..4448
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4375..4394
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4529..5323
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4583..4602)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5193..5212
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5497..5618
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5534..5553)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5588..5607
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5667..5683)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5667..5683)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5680..5702)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5691..5707
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5715..5721,5722..5739,5740..5745))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5760..5781
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5898..5915)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6069..6657)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6149..6168)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6828..7619,7620..7688))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7451..7470
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7689..7793)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
      961 agatagggac cttctgctga gccagctttg cttcctggcc tcggtggcgc tgctgctctg
     1021 gtcgctgtcg agcctgcgag agcagaagga acttgacctc atggatctca ttggggaaga
     1081 tagaaaatgg atggtgggga ggaagctgat gcaggtgaat gatacactga cttctgaaga
     1141 tgcagggctc cagagcagca agaactgcac agaaccagcc ctgcatgaat tccccaggga
     1201 catcttctcc aatgaagacc gacgacaggg agcagtggtc ctccacgtgc tctgtgccat
     1261 gtacatgttc tatgcgctgg ccattgtgtg cgatgacttc ttcgtcccat ccttggaaaa
     1321 gatctgtgag cgcctccacc tcagtgaaga tgtggcagga gccacattca tggcagcagg
     1381 cagctcggcc ccagagctgt tcacgtcggt cataggagtc ttcatcacaa agggtgatgt
     1441 gggagttggc accatcgtgg gctctgccgt gttcaacatc ctgtgcatca ttggcgtctg
     1501 cgggctcttt gcagggcagg tggtggctct ttcctcctgg tgcctgctga gggactccat
     1561 ctactacacg ctgtctgtgg tcgcactcat tgtgttcatt tacgatgaaa aagtttcttg
     1621 gtgggaatct ctagtccttg tgctgatgta cctcatctat atcgtcatca tgaaatacaa
     1681 tgcgtgcata caccagtgct tcgaaaggag gacgaaaggt gccgggaaca tggtcaacgg
     1741 attggccaac aatgcagaaa tcgacgacag cagcaactgc gatgccactg tggtgctact
     1801 caagaaagcc aatttccacc gcaaagcgtc cgtgatcatg gtggatgagc tgctgtctgc
     1861 ctacccacac cagctttctt tctctgaggc tggccttcga attatgatca caagccactt
     1921 tcctcccaag acccgcctct ccatggccag tcgcatgtta atcaatgaga gacaaagact
     1981 gataaacagc agggcctata ccaatgggga atctgaagtg gccatcaaaa tcccaattaa
     2041 gcacacagtg gagaacggaa cagggcccag cagtgcccca gacaggggtg tgaacggaac
     2101 ccggagggac gacatagttg ctgagactga caatgagaca gagaacgaga atgaagacaa
     2161 tgagaataac gagagtgatg aagaggagga ggaggatgaa gatgatgatg aaggccccta
     2221 cacgccgttc gaccccccct ccggcaaact ggaaactgtg aaatgggctt tcacctggcc
     2281 actgagcttt gtcttgtact tcactgtccc caactgtaac aaaccccact gggagaagtg
     2341 gtttatggtg acattcgctt cctctacgct atggatcgca gccttctcct acatgatggt
     2401 gtggatggtt accatcatcg gctacacact agggatccct gatgtcatca tggggatcac
     2461 cttcctggct gccggaacca gcgtgccaga ctgcatggcc agcctcattg tagccagaca
     2521 agggatgggg gatatggctg tgtccaattc cattgggagc aacgtctttg acatcttgat
     2581 tgggctcggt cttccctggg ctctgcagac tctggctgta gactacggat cctatattcg
     2641 gctgaatagc agagggctca tctactccgt gggcttgctc ctggcctctg tctttgtcac
     2701 ggtctttggt gttcacttga acaagtggca gctggacaag aagctgggct gtggatgcct
     2761 cttcctgtac ggcgtgttcc tctgtttctc catcatgacg gagttcaacg tgttcacctt
     2821 tgtgaacttg cccatgtgtg gggactaatg attggccagg ccacctgctg aggcccaggt
     2881 ccttcttttc tgtgcaatac gagacccggc cgcacccgag tcaggcggcc cctcaacagc
     2941 cgaggcatcc gtctctcccg tgctgtccac aggcctccgc tcctgtcctg gtggcctagg
     3001 ctctccctgc cacctccttt gctccccaac tccctcggcc ctacctccca gccggccccc
     3061 ctcacctgcc caccccattc ctgcctcctc ttccgtgtga agacatccaa catccacatg
     3121 aattttcaag ctccattttt gaacagtgac tgagattcta gaacaaaact ggctgctaac
     3181 tggcccaagc caggcacaac ttactccaat ccctcctcct tttttaagtt atttcacaga
     3241 ataccctaat ttatgaccag agactttgag gaaacaggag agggtccgct gatggcagga
     3301 aggtttgttt tgtttgggag tgagtcctag gttacggggc tcagggaaat tgtttaatcc
     3361 agcacagtgg cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac
     3421 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     3481 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     3541 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     3601 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     3661 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     3721 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     3781 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3841 tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3901 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3961 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4021 ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt
     4081 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     4141 ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa gcatgcatct
     4201 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4261 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     4321 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4381 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     4441 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     4501 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     4561 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     4621 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     4681 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     4741 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     4801 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     4861 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     4921 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     4981 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     5041 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     5101 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     5161 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     5221 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     5281 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     5341 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     5401 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     5461 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     5521 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5581 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     5641 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     5701 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     5761 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     5821 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     5881 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     5941 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6001 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6061 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6121 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6181 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6241 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
     6301 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6361 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     6421 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     6481 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     6541 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     6601 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     6661 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6721 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     6781 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     6841 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     6901 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     6961 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7021 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7081 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7141 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7201 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7261 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7321 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7381 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7441 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     7501 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     7561 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     7621 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     7681 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7741 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     7801 ccccgaaaag tgccacctga cgtc
//
