LOCUS       sequence-142850-        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses NET1A-V5 in mammalian cells in a doxycycline inducible 
            manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE,
            Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
  TITLE     Identification of AMPK Phosphorylation Sites Reveals a Network of 
            Proteins Involved in Cell Invasion and Facilitates Large-Scale 
            Substrate Prediction.
  JOURNAL   Cell Metab. 2015 Nov 3;22(5):907-21. doi: 
            10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8.
  PUBMED    26456332
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.09.009"; journalName: "Cell Metab"; date: 
            "2015-11-3- 3"; volume: "22"; issue: "5"; pages: "907-21"
COMMENT     Sequence Label: FUW TetO NET1A-V5 S46A
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(80..121)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    147..171
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcaatctttc acaaattttg taatccagag gttgattatc gataagcttg atatcgaatt
       61 agctagctta accggtacgc gtagaatcga gaccgaggag agggttaggg ataggcttac
      121 cttcgaaccg cgggccctct agatcaacca ctttgtacaa gaaagttggg tacaccaaag
      181 tctctttccg tttgccacca gaaagggctt tgtccctcgc tcttcggatg ccgggctgtg
      241 tctggtgtgt ctttaagctc ttgctgtcct ctgccatctg catgccagag ccacatctgt
      301 aagcgttttc atcaacttct acctgagtaa cactggaaac tgtggatgcc ctcctctggg
      361 ctgtgagttt cctcgcagag gggtggttcc cctcacactc ttcgtgcagc tccggcaggc
      421 cctgcagctc aggtggactg cctgccgact ggaagggggc aatggccgct cgaatacagt
      481 tgaaccactg ctgcttgtgg aacacgtcat tggcttgcag agtgtgagac tgggctggag
      541 aggggtcatg gaagcgaatt ctaaagatat ttttagcttt ctctgagtta ctgaaagctc
      601 ctcgaaagga gcctcccatt ctcacatctc catcctgcag gtcttctagg actagctctt
      661 ggactgggat tggctgccgg taaacctggt aagagtgccg ttcgttccgt gtgacgggcc
      721 gagtcagaac caagatgtct tgaaacagga aaatgtaaag tttatgtcca ctcttgctcc
      781 gcagctcccc atggcacagc agcactttgc tcgcttcgat tctggggtcc ctctgctttt
      841 catccaggta ctccagcttg tcgatgtaat actggcactc ggattcacct ttcttcaagt
      901 tgatatcaga gaggactccc tgtattatca atatagcatc ctccagaagc tgaacatcag
      961 ggtgctcttt tggagtgtgt ttaagaattt cttttaacag taaagggta
//
