LOCUS       sequence-142852-        9151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SLC24A3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9151)
  AUTHORS   Kraev A, Quednau BD, Leach S, Li XF, Dong H, Winkfein R, Perizzolo 
            M, Cai X, Yang R, Philipson KD, Lytton J
  TITLE     Molecular cloning of a third member of the potassium-dependent 
            sodium-calcium exchanger gene family, NCKX3.
  JOURNAL   J Biol Chem. 2001 Jun 22;276(25):23161-72. Epub 2001 Apr 9.
  PUBMED    11294880
REFERENCE   2  (bases 1 to 9151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2001-06-22"; volume: "276(25)"; pages: "23161-72. 
            Epub 2001 Apr 9"
COMMENT     Sequence Label: Human NCKX3-FlagTag (pcDNA3.1-)
FEATURES             Location/Qualifiers
     source          1..9151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1151..1174
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4745..4762)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4751..4975
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5021..5449
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5108..5127)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5318..5339
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5458..5478)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5463..5792
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5643..5778
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5705..5724
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5859..6653
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5913..5932)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6523..6542
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6827..6948
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6864..6883)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6918..6937
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6997..7013)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6997..7013)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7010..7032)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7021..7037
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7045..7051,7052..7069,7070..7075))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7090..7111
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7228..7245)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7399..7984)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7479..7498)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8155..8946,8947..9015))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8778..8797
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9016..9120)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatca ggatgcggcc
      961 gtccggcgac gaggaccgcg cgcgtcgccg ccgccgccgc cgccgccgga gggaccttct
     1021 gctgagccag ctctgcttcc tggcctcggt ggcgctgctg ctctggtcgc tgtcgagcct
     1081 gcgagagcag aaggagcttg acctcatgga cctcataggg gaagacagaa agtggatgat
     1141 ggcgaggaag gactacaagg acgacgatga caagctgatg caggtgaacg acactctgac
     1201 ttccgaagat gccggactcc ggaacagcaa gaactgcacc gaaccagccc tgcatgaatt
     1261 ccccaatgac atcttcacaa acgaggatag aagacaaggt gcggtggtcc tccatgtgct
     1321 ctgtgccata tacatgttct atgcgctggc cattgtgtgt gatgacttct tcgtcccttc
     1381 cttggaaaag atctgtgagc gcctgcacct cagtgaagat gtggctgggg ccacattcat
     1441 ggcagcggga agttcagccc cagagctgtt cacatcggtc ataggggtct tcatcaccaa
     1501 aggcgatgtg ggagttggca ccatcgtggg ctcagcggta ttcaacatcc tgtgcatcat
     1561 tggtgtctgt gggctctttg ctgggcaggt tgtggctctt tcctcctggt gcctactgag
     1621 ggattctatc tactacacgc tgtctgtgat cgcgctcatc gtgtttattt atgatgaaaa
     1681 agtttcctgg tgggagtctt tagtccttgt gctgatgtat cttatctaca ttgtcatcat
     1741 gaaatataac gcttgcatac atcagtgctt tgagaggagg acaaaaggtg ccgggaacat
     1801 ggtcaacgga ttggccaaca atgctgaaat tgatgacagc agcaactgcg acgcaactgt
     1861 ggtgctactt aagaaagcaa atttccaccg caaagcatca gtgatcatgg tagacgagct
     1921 gctgtcagcc tacccacacc agctttcctt ctctgaggct ggccttcgaa tcatgataac
     1981 cagccacttt ccccccaaga cccggctctc catggccagt cgcatgttga tcaatgagag
     2041 acaaagattg ataaacagca gggcttatac caacggggaa tctgaggtgg ccatcaaaat
     2101 cccaattaag cacaccgtgg agaatgggac agggcccagc agtgccccag acaggggcgt
     2161 gaatgggaca cggagggacg atgttgtggc tgaggctggc aacgaaacag agaatgaaaa
     2221 tgaggacaat gagaatgatg aggaggaaga ggaggacgag gatgatgatg aaggaccgta
     2281 cacaccattc gacaccccct cgggtaaact ggaaacagtg aaatgggcgt tcacctggcc
     2341 gctgagtttc gtcttatact tcactgtacc caactgcaac aagccgcgct gggagaaatg
     2401 gttcatggtg acgtttgctt cctccacgct gtggatcgca gccttctcct acatgatggt
     2461 gtggatggtc acaatcattg gttacaccct ggggattcct gacgtcatca tggggatcac
     2521 cttcctggct gctgggacca gcgtgcctga ctgcatggcc agcctcattg tggccagaca
     2581 agggatgggg gacatggctg tgtccaactc cattgggagc aacgtgtttg acatcctgat
     2641 tggcctcggt ctcccctggg ctctgcagac cctggctgtg gattacggat cctacatccg
     2701 gctgaatagc agggggctga tctactccgt aggcttgctc ctggcctctg tttttgtcac
     2761 ggtgttcggc gtccacctga acaagtggca gctggacaag aagctgggct gtgggtgcct
     2821 cctcctgtat ggtgtgttcc tgtgcttctc catcatgact gagttcaacg tgttcacctt
     2881 tgtgaacctg cccatgtgcg gggaccactg agccgccggg tgcccacaga ggctcagctc
     2941 cttcttttct gtgcaatacg agacccggcc gcaccccgag tcacacaggc ccccggggcc
     3001 acggcgttcg tctctcctgt gctgtcctca ggcctccgct cctgttttgg tggcccaggc
     3061 tctcccctga cccatcctcg ctcccccacc tccttgggtc atgcccaccc accctttcct
     3121 gcctcctccg tgtgaagaca tccaacatcc acgtgacttt tccagctcca tttttgaaca
     3181 gtgactgaga ttctagaaaa actggctgct aactggcctg agccaggcaa cactgattcc
     3241 aatccctcct ccttttttaa gttatttgat ggaagactca cctaatttgt gacctgagac
     3301 tgttgaagaa atagagagga gggggcccgt tgattacaga gagcatttgg gattttgttt
     3361 ggtttggaga tgatgcctag gttactgggt ttggggggat tgttttcttt tgggggcctt
     3421 ccccttttac tccttttctt ccagagatca agagcttctc ttgcatcttc ttccactggg
     3481 ctctggatta atcaattacc caaaggctgc acctgccgtg ttgtctgggc ttgcatccca
     3541 gatgtgttgg agtatgcatg gatgtagtgc tttttagagg agccactggg caaggccacc
     3601 aagaacaaat gcatgacatt ttatagccaa ggacgcctcg ctaaagtctt atgggcgtcc
     3661 cctggggttg ggggggcaca aggttttgga ggaagaagac aacttccctc attccatcat
     3721 caccatctct ttctcactag gttctttcta gttttcaagc aatagttcta gcctgccttg
     3781 gacaaggggg ccccagttaa acaaactacc catccatgag ctgccaggca gtcaaaaaca
     3841 gaagcttccc cgacttgtga gtccctgaga tgtgctcttg ttgtttggca tttggggtga
     3901 cagggagtga cccagaggcc accactgctt ttcatgcagg agttacagac actggtttct
     3961 tggaaaatgg agagaagcgc actttgcaca gacgtcgtca attaagtccc aatttgccac
     4021 ttggtattga gtacactgga ccctgaccac tggctcttgg gcaaacgtcc ttcctcacgg
     4081 ggcgcctccg ccaagccggc ccagctgcac ccctcccttc ctggagggat ggccagggaa
     4141 ggagaaaaca gagaactgac acctttgaaa ccacagaatg tgttacatgc agactcgctc
     4201 aagggcataa gttattgtga acgtttttgc caatcactgc tcaacagccc tgctagattt
     4261 tgtatgatgc tgaattatta tgcagactaa ttccacccag ttgagacaca ccatgcttgt
     4321 tcacttgtat ttattgaaac tgtggattct tgcccgtgct gtcccttgta tttactttaa
     4381 gcactgatca cttatcattc attcggtatg gttttccctg tcccttgtac acattctggt
     4441 atgaatttgt aaaaataacc tgctacaaat tggttgaatg tttctgtctg tggtgcgaac
     4501 cagcattaac ggatggggca cgtgcccaac tgaggaacag gagaagaaat caccaatttg
     4561 ggctctcaga gctaagacac acttattgat tctgttgcac attttgcact ggtttatggc
     4621 gattgttttc ttggacggat agtgtaaaat aaacttctct gttctctaaa aaaaaaaaaa
     4681 aaaagggcgg ccgctctaga ggatccgagc tcggtaccaa gcttaagttt aaaccgctga
     4741 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     4801 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     4861 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     4921 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     4981 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     5041 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5101 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5161 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5221 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5281 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5341 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5401 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     5461 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5521 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     5581 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     5641 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     5701 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     5761 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     5821 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     5881 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     5941 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     6001 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     6061 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     6121 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     6181 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     6241 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     6301 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     6361 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     6421 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     6481 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     6541 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     6601 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     6661 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     6721 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     6781 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     6841 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6901 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     6961 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7021 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7081 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7141 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     7201 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7261 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7321 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7381 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7441 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7501 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7561 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7621 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7681 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7741 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7801 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     7861 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7921 caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7981 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8041 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8101 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8161 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8221 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8281 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8341 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8401 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8461 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8521 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8581 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8641 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8701 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8761 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8821 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8881 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8941 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9001 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9061 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9121 cacatttccc cgaaaagtgc cacctgacgt c
//
