LOCUS       sequence-142858-        7332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SLC24A4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7332)
  AUTHORS   Li XF, Kraev AS, Lytton J
  TITLE     Molecular cloning of a fourth member of the potassium-dependent 
            sodium-calcium exchanger gene family, NCKX4.
  JOURNAL   J Biol Chem. 2002 Dec 13;277(50):48410-7. Epub 2002 Oct 11.
  PUBMED    12379639
REFERENCE   2  (bases 1 to 7332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-12-13"; volume: "277(50)"; pages: "48410-7. Epub
            2002 Oct 11"
COMMENT     Sequence Label: Human NCKX4-AS-long (pcDNA3.1+)
FEATURES             Location/Qualifiers
     source          1..7332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     934..950
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2875..2891)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2922..2939)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2928..3152
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3198..3626
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3285..3304)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3495..3516
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3635..3655)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3640..3970
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3821..3956
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3883..3902
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4037..4831
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4091..4110)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4701..4720
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5005..5126
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5042..5061)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5096..5115
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5175..5191)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5175..5191)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5188..5210)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5199..5215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5223..5229,5230..5247,5248..5253))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5268..5289
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5406..5423)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5577..6165)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5657..5676)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6336..7127,7128..7196))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6959..6978
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7197..7301)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga
      961 tctcaaagtt cgcaggaggc gagagatgct gccgcagcaa gtcggcttcg tgtgcgcggt
     1021 gctggccctg gtgtgctgtg cgtccggcct cttcggcagc ttggggcaca aaacagcttc
     1081 tgctagcaaa cgtgtcctgc cagacacatg gagaaataga aagttgatgg ccccagtgaa
     1141 tgggacacag acagccaaga actgcacaga tcctgcgatt cacgagttcc ccacagatct
     1201 gttctccaat aaggagcgac agcacggagc cgtcctgctg cacatccttg gtgctctgta
     1261 tatgttctat gccttggcca tagtgtgcga tgacttcttt gttccgtctc tagagaagat
     1321 ctgtgagaga ctccatctga gcgaagatgt ggctggagcc accttcatgg ctgcaggaag
     1381 ctcaacgcca gagctgtttg cgtctgttat tggggtgttc atcacccatg gggacgtcgg
     1441 ggtgggcacc atcgtgggct ctgctgtgtt caacatcctg tgcataattg gagtgtgcgg
     1501 actgtttgct ggccaggtgg tccgtctgac gtggtgggcc gtgtgccgag actccgtgta
     1561 ctacaccatc tctgtcatcg tgctcatcgt gttcatatat gatgaacaaa ttgtgtggtg
     1621 ggaaggcctg gtgctcatca tcttgtatgt gttttatatt ctgatcatga agtacaatgt
     1681 gaagatgcaa gcctttttca cagtcaaaca aaagagcatt gcaaacggta acccggtcaa
     1741 cagtgagctg gaggctggta atgatttcta tgacggtagc tatgatgacc cttccgtgcc
     1801 attgctgggg caagtgaagg agaagccaca gtatggcaag aaccccgtgg tgatggtgga
     1861 cgagattatg agctccagcc ctcccaagtt caccttccct gaagcaggct tacgaatcat
     1921 gatcaccaat aagtttggac ctaggacccg actacggatg gccagcagga tcatcattaa
     1981 tgagcggcag agactgatca actcggccaa tggtgtgagc agtaagccgc ttcaaaacgg
     2041 gaggcacgag aacattgaga acgggaatgt tcctgtggaa aaccccgaag accctcagca
     2101 gaatcaggag cagcagccgc cgccacagcc accaccgcca gagccagagc cggtggaggc
     2161 tgacttcctg tcccccttct ccgtgccgga ggccagaggg gacaaggtca agtgggtgtt
     2221 cacctggccc ctcatcttcc tcctgtgcgt caccattccc aactgcagca agccccgctg
     2281 ggagaagttc ttcatggtca ccttcatcac cgccacgctg tggatcgctg tgttctccta
     2341 catcatggtg tggctggtga ctattatcgg atacacactt gggatcccgg atgtcatcat
     2401 gggcattact ttcctggcag cagggacaag tgttccagac tgcatggcca gcctaattgt
     2461 ggcgagacaa ggccttgggg acatggcagt ctccaacacc ataggaagca acgtgtttga
     2521 catcctggta ggacttggtg taccgtgggg cctgcagacc atggttgtta attatggatc
     2581 aacagtgaag atcaacagcc gggggctggt ctattccgtg gtcctgttgc tgggctctgt
     2641 cgctctcacc gtcctcggca tccacctaaa caagtggcga ctggaccgga agctgggtgt
     2701 ctacgtgctg gttctctacg ccatcttctt gtgcttctcc ataatgatag agtttaacgt
     2761 ctttaccttc gtcaacttgc cgatgtgccg ggaagacgat tagcgctgag tcgcggcccc
     2821 tgggagctga tctggacacc ctgtgacact ggcgttctcc tctatcaagc ttatcgatac
     2881 cgtcgacctc gagggggggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     2941 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     3001 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     3061 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     3121 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     3181 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     3241 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     3301 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc ggggcatccc
     3361 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     3421 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     3481 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     3541 ctattctttt gatttataag ggattttggg gatttcggcc tattggttaa aaaatgagct
     3601 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     3661 aagtccccag gctccccagg caggcagaag tatgcaaagc atgcatctca attagtcagc
     3721 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3781 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     3841 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     3901 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     3961 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     4021 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     4081 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     4141 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     4201 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     4261 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     4321 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     4381 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     4441 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     4501 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     4561 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     4621 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     4681 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     4741 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     4801 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     4861 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     4921 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     4981 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     5041 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     5101 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     5161 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     5221 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     5281 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     5341 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     5401 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     5461 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     5521 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     5581 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5641 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     5701 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     5761 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     5821 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     5881 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     5941 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6001 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     6061 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6121 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6181 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6241 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     6301 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     6361 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     6421 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     6481 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     6541 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     6601 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     6661 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     6721 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     6781 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     6841 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     6901 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     6961 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     7021 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     7081 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     7141 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     7201 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     7261 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     7321 ccacctgacg tc
//
