LOCUS       sequence-142861-        7072 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SLC24A5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7072)
  TITLE     Lytton lab plasmids
REFERENCE   2  (bases 1 to 7072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Mouse NCKX5 (pcDNA3.1+)
FEATURES             Location/Qualifiers
     source          1..7072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     986..1002
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     987..1003
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2601..2617)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2601..2617)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2662..2679)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2668..2892
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2938..3366
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3025..3044)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3235..3256
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3375..3395)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3380..3710
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3561..3696
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3623..3642
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3777..4571
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3831..3850)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4441..4460
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4745..4866
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4782..4801)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4836..4855
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4915..4931)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4915..4931)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4928..4950)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4939..4955
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4963..4969,4970..4987,4988..4993))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5008..5029
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5146..5163)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5317..5905)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5397..5416)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6076..6867,6868..6936))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6699..6718
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6937..7041)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
      961 agatatccag cacagtggcg gccgctcgag gtcgacggta tcgataagct tgatatcgaa
     1021 ttcccaaaga gcacgctgga aatgcggaca aaatggggcc caacttggac aaggagggtc
     1081 ctgctgctgg ggatcttttg ggtctctgca tacctgcctg tccgtggtgt ctccctgccc
     1141 ccgcgcctcc caagggccac aggaaacagc acgcaatgtg ctgtctctcc agcatcggag
     1201 tttcccgaag ggttcttcac caagcaggag agcacggatg gaggtatcgt aatctacttc
     1261 ctcattatcc tctacatgtg catggccatc tccatcgtct gcgacaagta cttcttgccc
     1321 tccctggaaa tcatcagtga ctcccttgga ctgtcgcagg atgttgcagg tgcaactttc
     1381 atggcagctg gtagctccgc ccctgaacta gttaccgcct tcttgggtgt atttatcaca
     1441 aagggagata ttggtatcag caccatacta ggatctgcaa tttataatct cctcggcatc
     1501 tgtgctgcct gcggcctcct gtccaacatg gtttcaacac tctcctgctg gcccctcttc
     1561 cgagactgtg cagtgtatgc agttagtgta ggagcagttt ttggcataat atttgacaac
     1621 cgaatttact ggtacgaagg agcagggctg ctactgatat atgggctcta tgttctgctg
     1681 ctgtgttttg acaccacgat cagccggcat gtcatgaaaa catgcagtcc ctgttgtccc
     1741 tgccttgcca gagctatgga ggagagaatt gaacagcaga cactgttggg atgggaggat
     1801 gagagccagc tcttcattcg tcgccagtca aggacagaca gtggaatatt tcaggaagat
     1861 tccggttact cccaactttc tctaagcttg catggtctta gtcaggtttc tgaagatcca
     1921 ccaagtgttt tcagcatgcc agaagcagac ttaagaagaa ttttttgggt tctctctctt
     1981 cctattatta cattacttgc tctgacaaca ccagactgca ggagaaagtt ttggaaaaat
     2041 tactttgtga taaccttttt catgtctgca ctatggatat ctgcattcac atacatccta
     2101 gtttggatgg tcacagtaac aggggaaaca ctagggatcc cagacacagt gatgggcctt
     2161 actttgttgg cagcagggac aagcatacca gatacagtca caagtgtgtt agttgcaaga
     2221 aaaggtaaag gagacatggc tatatctaac atcgtgggat ccaatgtgtt tgatatgcta
     2281 tgcctaggtc ttccctggtt tattaagact gcatttacga atgcatctgc tcctatagaa
     2341 gtgaatagca agggactcac atatataaca atctctctca acatttcaat tttatttctg
     2401 ttcttagcag ttcattttaa tggctggaaa ctagaccgaa agttgggggt ggtctgccta
     2461 gtgttatact tgggacttgc tactttatca gttctatatg aaattggaat tattggaaat
     2521 aatagaataa ggggttgtgg agtttgatat tttttaatag tgttatgtgg aaagatggaa
     2581 ggaattcctg cagcccgggg gatccactag ttctagagcg gccgctcgag tctagagggc
     2641 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     2701 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     2761 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     2821 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     2881 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     2941 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     3001 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     3061 tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg
     3121 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     3181 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     3241 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     3301 ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     3361 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg
     3421 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     3481 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     3541 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3601 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     3661 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     3721 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     3781 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     3841 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     3901 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     3961 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     4021 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     4081 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     4141 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     4201 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     4261 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     4321 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     4381 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     4441 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     4501 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     4561 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     4621 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     4681 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     4741 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     4801 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     4861 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     4921 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     4981 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     5041 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     5101 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     5161 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     5221 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     5281 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     5341 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     5401 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     5461 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
     5521 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     5581 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     5641 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     5701 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     5761 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     5821 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     5881 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     5941 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     6001 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     6061 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     6121 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     6181 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     6241 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     6301 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     6361 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     6421 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     6481 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     6541 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     6601 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     6661 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     6721 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     6781 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     6841 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     6901 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     6961 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     7021 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
