LOCUS       sequence-14287-e        8568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8568)
  AUTHORS   Werdien D, Peiler G, Ryffel GU
  TITLE     FLP and Cre recombinase function in Xenopus embryos.
  JOURNAL   Nucleic Acids Res. 2001 Jun 1. 29(11):E53-3.
  PUBMED    11376165
REFERENCE   2  (bases 1 to 8568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2001 Jun 1. 29(11):E53-3."
COMMENT     Sequence Label: pCMV:GFP(loxP)lacZ
FEATURES             Location/Qualifiers
     source          1..8568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     protein_bind    complement(176..209)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        217..235
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             276..992
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="MSKGEELFTGVVPILVELDGDVNGYKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDDPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(306..334)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     929..949
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     polyA_signal    1114..1248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(1250..1283)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      1289..1298
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1315..1332)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1315..1331)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1322..4369
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(1324..1346)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     intron          4464..4529
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    4576..4710
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4864..4884)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4864..4882)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4903..4919)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4903..4919)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4916..4938)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4927..4943
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4951..4957,4958..4975,4976..4981))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4996..5017
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5134..5151)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5305..5893)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5385..5404)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6064..6855,6856..6924))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6687..6706
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6925..7029)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7055..7510)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7192..7213)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7404..7423
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7584..8568
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atccatcgat tcccgggaat tcaggcctct cgagcctcta
      121 ggaacagtcg acgacactgc agagacctac ttcactaaca accggtacag ttcgaataac
      181 ttcgtatagc atacattata cgaagttata agcttgattt aggtgacact atagaataca
      241 agctacttgt tctttttgca ggatccacag ccaccatgag taaaggagaa gaacttttca
      301 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggtac aaattttctg
      361 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
      421 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactctcact tatggtgttc
      481 aatgcttttc aagataccca gatcatatga agcggcacga cttcttcaag agcgccatgc
      541 ctgagggata cgtgcaggag aggaccatct tcttcaagga cgacgggaac tacaagacac
      601 gtgctgaagt caagtttgag ggagacaccc tcgtcaacag gatcgagctt aagggaatcg
      661 atttcaagga ggacggaaac atcctcggcc acaagttgga atacaactac aactcccaca
      721 acgtatacat catggccgac aagcagaaga acggcatcaa agccaacttc aagacccgcc
      781 acaacatcga agacggcggc gtgcaactcg ctgatcatta tcaacaaaat actccaattg
      841 gcgatgaccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga
      901 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga
      961 ttacacatgg catggatgaa ctatacaaat aactcgagcc tctagaggat cataatcagc
     1021 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     1081 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     1141 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     1201 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcca taacttcgta
     1261 tagcatacat tatacgaagt tataccgggc caccatggtc gcgagtagct tggcactggc
     1321 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     1381 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     1441 ccaacagttg cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc
     1501 ggtgccggaa agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc
     1561 aaactggcag atgcacggtt acgatgcgcc catctacacc aacgtaacct atcccattac
     1621 ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa
     1681 tgttgatgaa agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttaactc
     1741 ggcgtttcat ctgtggtgca acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc
     1801 gtctgaattt gacctgagcg catttttacg cgccggagaa aaccgcctcg cggtgatggt
     1861 gctgcgttgg agtgacggca gttatctgga agatcaggat atgtggcgga tgagcggcat
     1921 tttccgtgac gtctcgttgc tgcataaacc gactacacaa atcagcgatt tccatgttgc
     1981 cactcgcttt aatgatgatt tcagccgcgc tgtactggag gctgaagttc agatgtgcgg
     2041 cgagttgcgt gactacctac gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc
     2101 cagcggcacc gcgcctttcg gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg
     2161 cgtcacacta cgtctgaacg tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct
     2221 ctatcgtgcg gtggttgaac tgcacaccgc cgacggcacg ctgattgaag cagaagcctg
     2281 cgatgtcggt ttccgcgagg tgcggattga aaatggtctg ctgctgctga acggcaagcc
     2341 gttgctgatt cgaggcgtta accgtcacga gcatcatcct ctgcatggtc aggtcatgga
     2401 tgagcagacg atggtgcagg atatcctgct gatgaagcag aacaacttta acgccgtgcg
     2461 ctgttcgcat tatccgaacc atccgctgtg gtacacgctg tgcgaccgct acggcctgta
     2521 tgtggtggat gaagccaata ttgaaaccca cggcatggtg ccaatgaatc gtctgaccga
     2581 tgatccgcgc tggctaccgg cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg
     2641 taatcacccg agtgtgatca tctggtcgct ggggaatgaa tcaggccacg gcgctaatca
     2701 cgacgcgctg tatcgctgga tcaaatctgt cgatccttcc cgcccggtgc agtatgaagg
     2761 cggcggagcc gacaccacgg ccaccgatat tatttgcccg atgtacgcgc gcgtggatga
     2821 agaccagccc ttcccggctg tgccgaaatg gtccatcaaa aaatggcttt cgctacctgg
     2881 agagacgcgc ccgctgatcc tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg
     2941 tttcgctaaa tactggcagg cgtttcgtca gtatccccgt ttacagggcg gcttcgtctg
     3001 ggactgggtg gatcagtcgc tgattaaata tgatgaaaac ggcaacccgt ggtcggctta
     3061 cggcggtgat tttggcgata cgccgaacga tcgccagttc tgtatgaacg gtctggtctt
     3121 tgccgaccgc acgccgcatc cagcgctgac ggaagcaaaa caccagcagc agtttttcca
     3181 gttccgttta tccgggcaaa ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga
     3241 taacgagctc ctgcactgga tggtggcgct ggatggtaag ccgctggcaa gcggtgaagt
     3301 gcctctggat gtcgctccac aaggtaaaca gttgattgaa ctgcctgaac taccgcagcc
     3361 ggagagcgcc gggcaactct ggctcacagt acgcgtagtg caaccgaacg cgaccgcatg
     3421 gtcagaagcc gggcacatca gcgcctggca gcagtggcgt ctggcggaaa acctcagtgt
     3481 gacgctcccc gccgcgtccc acgccatccc gcatctgacc accagcgaaa tggatttttg
     3541 catcgagctg ggtaataagc gttggcaatt taaccgccag tcaggctttc tttcacagat
     3601 gtggattggc gataaaaaac aactgctgac gccgctgcgc gatcagttca cccgtgcacc
     3661 gctggataac gacattggcg taagtgaagc gacccgcatt gaccctaacg cctgggtcga
     3721 acgctggaag gcggcgggcc attaccaggc cgaagcagcg ttgttgcagt gcacggcaga
     3781 tacacttgct gatgcggtgc tgattacgac cgctcacgcg tggcagcatc aggggaaaac
     3841 cttatttatc agccggaaaa cctaccggat tgatggtagt ggtcaaatgg cgattaccgt
     3901 tgatgttgaa gtggcgagcg atacaccgca tccggcgcgg attggcctga actgccagct
     3961 ggcgcaggta gcagagcggg taaactggct cggattaggg ccgcaagaaa actatcccga
     4021 ccgccttact gccgcctgtt ttgaccgctg ggatctgcca ttgtcagaca tgtatacccc
     4081 gtacgtcttc ccgagcgaaa acggtctgcg ctgcgggacg cgcgaattga attatggccc
     4141 acaccagtgg cgcggcgact tccagttcaa catcagccgc tacagtcaac agcaactgat
     4201 ggaaaccagc catcgccatc tgctgcacgc ggaagaaggc acatggctga atatcgacgg
     4261 tttccatatg gggattggtg gcgacgactc ctggagcccg tcagtatcgg cggaattcca
     4321 gctgagcgcc ggtcgctacc attaccagtt ggtctggtgt caaaaataat aataaccggg
     4381 caggggggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac
     4441 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac
     4501 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc
     4561 agtggtggaa tgccagatcc agacatgata agatacattg atgagtttgg acaaaccaca
     4621 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     4681 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     4741 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     4801 atggctgatt atgatctgcg gccgcggcgc caatgcattg ggcccggtac ccagcttttg
     4861 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     4921 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     4981 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     5041 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     5101 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     5161 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5221 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5281 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5341 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5401 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5461 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     5521 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5581 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5641 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5701 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     5761 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5821 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5881 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5941 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     6001 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     6061 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     6121 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     6181 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     6241 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     6301 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     6361 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     6421 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     6481 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     6541 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6601 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6661 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6721 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     6781 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     6841 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     6901 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     6961 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     7021 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
     7081 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     7141 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     7201 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     7261 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     7321 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     7381 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     7441 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     7501 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     7561 gcctcttcgc tattacgcca gtcgaccata gccaattcaa tatggcgtat atggactcat
     7621 gccaattcaa tatggtggat ctggacctgt gccaattcaa tatggcgtat atggactcgt
     7681 gccaattcaa tatggtggat ctggacccca gccaattcaa tatggcggac ttggcaccat
     7741 gccaattcaa tatggcggac ttggcactgt gccaactggg gaggggtcta cttggcacgg
     7801 tgccaagttt gaggaggggt cttggccctg tgccaagtcc gccatattga attggcatgg
     7861 tgccaataat ggcggccata ttggctatat gccaggatca atatataggc aatatccaat
     7921 atggccctat gccaatatgg ctattggcca ggttcaatac tatgtattgg ccctatgcca
     7981 tatagtattc catatatggg ttttcctatt gacgtagata gcccctccca atgggcggtc
     8041 ccatatacca tatatggggc ttcctaatac cgcccatagc cactccccca ttgacgtcaa
     8101 tggtctctat atatggtctt tcctattgac gtcatatggg cggtcctatt gacgtatatg
     8161 gcgcctcccc cattgacgtc aattacggta aatggcccgc ctggctcaat gcccattgac
     8221 gtcaatagga ccacccacca ttgacgtcaa tgggatggct cattgcccat tcatatccgt
     8281 tctcacgccc cctattgacg tcaatgacgg taaatggccc acttggcagt acatcaatat
     8341 ctattaatag taacttggca agtacattac tattggaagt acgccagggt acattggcag
     8401 tactcccatt gacgtcaatg gcggtaaatg gcccgcgatg gctgccaagt acatccccat
     8461 tgacgtcaat ggggaggggc aatgacgcaa atgggcgttc cattgacgta aatgggcggt
     8521 aggcgtgcct aatgggaggt ctatataagc aatgctcgtt tagggaac
//
