LOCUS       sequence-142892-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: GalT Targeting Sequence, Excitation: 433, Emission: 
            475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCerulean-Golgi-7
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      280..289
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(286..288,289..291,292..804)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(331..352)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(592..611)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 accgtcagat ccgctagcat gaggcttcgg gagccgctcc tgagcggcag cgccgcgatg
       61 ccaggcgcgt ccctacagcg ggcctgccgc ctgctcgtgg ccgtctgcgc tctgcacctt
      121 ggcgtcaccc tcgtttacta cctggctggc cgcgacctga gccgcctgcc ccaactggtc
      181 ggagtctcca caccgctgca gggcggctcg aacagtgccg ccgccatcgg gcagtcctcc
      241 ggggagctcc ggaccggagg ggccaaggat ccaccggtcg ccaccatggt gagcaagggc
      301 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      361 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      421 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      481 acctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
      541 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      601 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      661 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      721 gccatcagcg acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
      781 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccga
//
