LOCUS       sequence-142898-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains GFPmut3b under the Ptrc promoter. Suicide vector inserts 
            into slr0168 site of Synechocystis. Carries chloramphenicol 
            resistance. Propogates in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Yao L, Cengic I, Anfelt J, Hudson EP
  TITLE     Multiple Gene Repression in Cyanobacteria Using CRISPRi.
  JOURNAL   ACS Synth Biol. 2015 Dec 28.
  PUBMED    26689101
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Dec 28."
COMMENT     Sequence Label: pMD19T-slr0168-Ptrc-GFP3mut-CmR
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    18..38
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 ggcagggcgg ggcgtaatac tagtagcggc cgctgcagga ttattcggcg gtttataatc
       61 ccggtggccc cggcaatgat ccagagaatg gtcccccagg gccctttact gtgtccagta
      121 gtccccaggt aattgaggta acggatacca tcggccagcc caccaaagtc tcctatgtgg
      181 aagtggatgg ccccgtattg cgtaatccct tcagtggtac tcccattggg caagaggtgg
      241 gtttagcggt taaagatctg gccacaggtc atgaaattta tcagtacact gacccagatg
      301 ggaaggtatt ttatgcttcc tttgctgccg ctgatgacca agccacggat ttaaccacgg
      361 cgatcgccaa tcccacggcc atcgatttaa ttaacgccag gggatttacg gcgggtagtt
      421 ccgtcaccgt atcgggttcc tacagtcggg aagccttttt tgatggatcc atgggttttt
      481 atcgacttct ggacgataac ggtgcagtgc tagatccctt aacaggtggt gtaatcaacc
      541 caggacaggt aggttatcaa gaagcagctt tggcagatag caatcgtttg caagccactg
      601 gctccaccct aacggcagaa gacctagaaa ccagagcatt ttccttcaat attttgggtg
      661 gcgagttgta tgcgccattt ttaacggtta atgacagtct ttccggtatt aatcagactt
      721 attttgcctt tgggtcggcc aacccagatg gcatcagcca cagcacaaac ttgggaccca
      781 acgtgattgg ttttgaagat tttctcggcg gaggagacct agatttcgac gacatcattg
      841 tcagatttac gctgacttag ctcaggggc
//
