LOCUS       sequence-142901-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton/Membrane, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Merlin-N-7
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(121..777)
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     regulatory      774..783
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
       61 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctagag tcgcggccgc
      121 ttagctatga gctactgcat cttctacctg ctcagtaatg ttgccttcgg ttttcctgac
      181 gaggcgatgg ccgatgtaat ggtctcctgg catttcaaga atctcttttg ccgccttgta
      241 agtagtcttc atttggcatt tgtgattgcc gcctccttca agttttaggt acatcgtaac
      301 atcacccttc agaactccgt cgctggcagt aattttctcg gttgatggct cccaatcaac
      361 actttggttt tgcatgatag gaccatcggc aggaaagtta accccagtaa atttggattt
      421 gtggtagaag gtgtttcctc taaggcttat atgcgcactg actgaagcgg acccaccatc
      481 ttcgaactcc aacgaccttt cccatgacag gccttcagga aatgcttgtt tgaaatagtc
      541 tggtatctct tctggatatt tagtaaatac tctgtggccg taacagaaca cgtgtgacac
      601 taagtcaaac gcgaaaggca ttggcccgcc ctcggccatt gtgacgcgta gtgtcatctc
      661 ttgatgtccc tcgtaaggtc tgcctgtgcc ttcaccttca attgtgaact catgcccatt
      721 gacggagccg tccatgtagt acctcatctt catctctggt ttaatcacac tcaccatggt
      781 ggcgaccggt ggatcccgga gctcttcaaa gaaggccact cgggacttgg cgctctgcaa
      841 ggtgagcttt ttaatggtat tgtgcttgct gctgccaccc ctgtcggagt tctcattgtg
      901 cagaatatcc agagctgtct ccctctctt
//
