LOCUS       sequence-142908-       10480 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of WT-STAT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10480)
  AUTHORS   Cheon H, Stark GR
  TITLE     Unphosphorylated STAT1 prolongs the expression of interferon-induced
            immune regulatory genes.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9373-8. doi: 
            10.1073/pnas.0903487106. Epub 2009 May 28.
  PUBMED    19478064
REFERENCE   2  (bases 1 to 10480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0903487106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-06-9- 9"; volume: "106"; issue: "23"; pages: "9373-8"
COMMENT     Sequence Label: pLV-WT-STAT1
FEATURES             Location/Qualifiers
     source          1..10480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    55..76
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(91..96,97..114,115..121)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    129..145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     134..156
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     153..169
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     153..169
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     188..208
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        190..208
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        236..462
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             463..643
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    690..815
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1308..1541
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1726..1770
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2037..2153
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2199..2217
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2241..2259
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2283..2301
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2325..2343
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2367..2385
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     enhancer        2426..2729
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2730..2933
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2884..2904
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        2978..2996
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5350..5373
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        5451..5808
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5451..5642
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5474..5494)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5659..5794
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5818..6417
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5818..5837)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6314..6334
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6473..6489)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6474..6490)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    6492..7080
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6545..6565)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6963..6974)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7209..7242)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7268..7448
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7520..7641
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7557..7576)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7611..7630
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(7856..7874)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7884..7901)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7884..7900)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7893..7915)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8042..8497
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8129..8148)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8339..8360
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8523..8627
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8628..8696,8697..9488)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8846..8865)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9659..10247
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10148..10167
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10401..10418
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
       61 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      181 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
      241 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
      301 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
      361 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
      421 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
      481 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
      541 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
      601 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
      661 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
      721 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
      781 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
      841 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
      901 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
      961 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     1021 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     1081 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     1141 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     1201 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     1261 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     1321 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     1381 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     1441 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     1501 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     1561 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     1621 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     1681 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     1741 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     1801 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     1861 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     1921 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     1981 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt
     2041 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     2101 acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcgatt
     2161 atccagccct cactccttcg atcactcgag tttaccactc cctatcagtg atagagaaaa
     2221 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     2281 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2341 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2401 ctcggtaccc gggtcgagag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     2461 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     2521 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     2581 cagtacatca agtgtgtcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     2641 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     2701 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2761 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2821 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2881 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
     2941 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
     3001 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattctg
     3061 cagatatcca tcacactggc ggccgcacaa ggtggcagga tgtctcagtg gtacgaactt
     3121 cagcagcttg actcaaaatt cctggagcag gttcaccagc tttatgatga cagttttccc
     3181 atggaaatca gacagtacct ggcacagtgg ttagaaaagc aagactggga gcacgctgcc
     3241 aatgatgttt catttgccac catccgtttt catgacctcc tgtcacagct ggatgatcaa
     3301 tatagtcgct tttctttgga gaataacttc ttgctacagc ataacataag gaaaagcaag
     3361 cgtaatcttc aggataattt tcaggaagac ccaatccaga tgtctatgat catttacagc
     3421 tgtctgaagg aagaaaggaa aattctggaa aacgcccaga gatttaatca ggctcagtcg
     3481 gggaatattc agagcacagt gatgttagac aaacagaaag agcttgacag taaagtcaga
     3541 aatgtgaagg acaaggttat gtgtatagag catgaaatca agagcctgga agatttacaa
     3601 gatgaatatg acttcaaatg caaaaccttg cagaacagag aacacgagac caatggtgtg
     3661 gcaaagagtg atcagaaaca agaacagctg ttactcaaga agatgtattt aatgcttgac
     3721 aataagagaa aggaagtagt tcacaaaata atagagttgc tgaatgtcac tgaacttacc
     3781 cagaatgccc tgattaatga tgaactagtg gagtggaagc ggagacagca gagcgcctgt
     3841 attggggggc cgcccaatgc ttgcttggat cagctgcaga actggttcac tatagttgcg
     3901 gagagtctgc agcaagttcg gcagcagctt aaaaagttgg aggaattgga acagaaatac
     3961 acctacgaac atgaccctat cacaaaaaac aaacaagtgt tatgggaccg caccttcagt
     4021 cttttccagc agctcattca gagctcgttt gtggtggaaa gacagccctg catgccaacg
     4081 caccctcaga ggccgctggt cttgaagaca ggggtccagt tcactgtgaa gttgagactg
     4141 ttggtgaaat tgcaagagct gaattataat ttgaaagtca aagtcttatt tgataaagat
     4201 gtgaatgaga gaaatacagt aaaaggattt aggaagttca acattttggg cacgcacaca
     4261 aaagtgatga acatggagga gtccaccaat ggcagtctgg cggctgaatt tcggcacctg
     4321 caattgaaag aacagaaaaa tgctggcacc agaacgaatg agggtcctct catcgttact
     4381 gaagagcttc actcccttag ttttgaaacc caattgtgcc agcctggttt ggtaattgac
     4441 ctcgagacga cctctctgcc cgttgtggtg atctccaacg tcagccagct cccgagcggt
     4501 tgggcctcca tcctttggta caacatgctg gtggcggaac ccaggaatct gtccttcttc
     4561 ctgactccac catgtgcacg atgggctcag ctttcagaag tgctgagttg gcagttttct
     4621 tctgtcacca aaagaggtct caatgtggac cagctgaaca tgttgggaga gaagcttctt
     4681 ggtcctaacg ccagccccga tggtctcatt ccgtggacga ggttttgtaa ggaaaatata
     4741 aatgataaaa attttccctt ctggctttgg attgaaagca tcctagaact cattaaaaaa
     4801 cacctgctcc ctctctggaa tgatgggtgc atcatgggct tcatcagcaa ggagcgagag
     4861 cgtgccctgt tgaaggacca gcagccgggg accttcctgc tgcggttcag tgagagctcc
     4921 cgggaagggg ccatcacatt cacatgggtg gagcggtccc agaacggagg cgaacctgac
     4981 ttccatgcgg ttgaacccta cacgaagaaa gaactttctg ctgttacttt ccctgacatc
     5041 attcgcaatt acaaagtcat ggctgctgag aatattcctg agaatcccct gaagtatctg
     5101 tatccaaata ttgacaaaga ccatgccttt ggaaagtatt actccaggcc aaaggaagca
     5161 ccagagccaa tggaacttga tggccctaaa ggaactggat atatcaagac tgagttgatt
     5221 tctgtgtctg aagttcaccc ttctagactt cagaccacag acaacctgct ccccatgtct
     5281 cctgaggagt ttgacgaggt gtctcggata gtgggctctg tagaattcga cagtatgatg
     5341 aacacagtag actacaagga cgacgatgac aagtaggcta gcggatccga attcggcgca
     5401 gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
     5461 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     5521 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     5581 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     5641 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5701 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     5761 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttaccatg
     5821 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     5881 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     5941 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     6001 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     6061 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     6121 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     6181 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     6241 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     6301 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     6361 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc
     6421 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcgataccg
     6481 tcgacctcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6541 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6601 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6661 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6721 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6781 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6841 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     6901 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6961 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7021 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7081 ctggaattaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     7141 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc cacgaagaca
     7201 agaattgaat aacttcgtat agcatacatt atacgaagtt ataccgattc tgctttttgc
     7261 ttctactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7321 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7381 tctgttgtgt gactctggta actagagatc cctcagaccc tttcagtcag tgtggaaaat
     7441 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7501 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     7561 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7621 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     7681 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7741 tttatttatg cagaggccga ggccgcctca tcgatgcggc cgcgcctcgg cctctgagct
     7801 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     7861 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     7921 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     7981 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     8041 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     8101 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     8161 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     8221 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     8281 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     8341 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     8401 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8461 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     8521 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8581 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     8641 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8701 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8761 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8821 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8881 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8941 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     9001 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     9061 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     9121 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     9181 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     9241 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     9301 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     9361 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     9421 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     9481 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9541 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9601 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9661 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9721 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9781 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9841 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9901 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9961 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    10021 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    10081 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    10141 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    10201 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    10261 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    10321 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    10381 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    10441 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
//
