LOCUS       sequence-142910-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8CeGFP
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(52..765)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(92..112)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     707..735
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgtaatacga ctcactatag ttctagaggc tcgagaggcc ttgaattctt atttgtatag
       61 ttcatccatg ccatgtgtaa tcccagcagc tgttacaaac tcaagaagga ccatgtggtc
      121 tctcttttcg ttgggatctt tcgaaagggc agattgtgtg gacaggtaat ggttgtctgg
      181 taaaaggaca gggccatcgc caattggagt attttgttga taatggtctg ctagttgaac
      241 gcttccatct tcaatgttgt gtctaatttt gaagttaact ttgattccat tcttttgttt
      301 gtctgccatg atgtatacat tgtgtgagtt atagttgtat tccaatttgt gtccaagaat
      361 gtttccatct tctttaaaat caataccttt taactcgatt ctattaacaa gggtatcacc
      421 ttcaaacttg acttcagcac gtgtcttgta gttcccgtca tctttgaaaa atatagttct
      481 ttcctgtaca taaccttcgg gcatggcact cttgaaaaag tcatgccgtt tcatatgatc
      541 tgggtatctt gaaaagcatt gaacaccata agtgagagta gtgacaagtg ttggccatgg
      601 aacaggtagt tttccagtag tgcaaataaa tttaagggta agttttccgt atgttgcatc
      661 accttcaccc tctccactga cagaaaattt gtgcccatta acatcaccat ctaattcaac
      721 aagaattggg acaactccag tgaaaagttc ttctccttta ctcatcaatc gatgtccggc
      781 gatcgctggc cggcctttaa ttaatggcgc gccactagtg gatcctgcaa a
//
