LOCUS       sequence-14292-e        5342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Werdien D, Peiler G, Ryffel GU
  TITLE     FLP and Cre recombinase function in Xenopus embryos.
  JOURNAL   Nucleic Acids Res. 2001 Jun 1. 29(11):E53-3.
  PUBMED    11376165
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2001 Jun 1. 29(11):E53-3."
COMMENT     Sequence Label: pCSFLPe
FEATURES             Location/Qualifiers
     source          1..5342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             94..1362
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIASCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     polyA_signal    1391..1525
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1415..1434)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1469..1488
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1638..1658)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1638..1656)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1677..1693)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1677..1693)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1690..1712)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1701..1717
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1725..1731,1732..1749,1750..1755))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1770..1791
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1908..1925)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2079..2667)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2159..2178)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2838..3629,3630..3698))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3461..3480
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3699..3803)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3829..4284)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3966..3987)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4178..4197
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4358..5342
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atccacagcc accatgccac aatttgatat attatgtaaa
      121 acaccaccta aggtcctggt tcgtcagttt gtggaaaggt ttgaaagacc ttcaggggaa
      181 aaaatagcat catgtgctgc tgaactaacc tatttatgtt ggatgattac tcataacgga
      241 acagcaatca agagagccac attcatgagc tataatacta tcataagcaa ttcgctgagt
      301 ttcgatattg tcaacaaatc actccagttt aaatacaaga cgcaaaaagc aacaattctg
      361 gaagcctcat taaagaaatt aattcctgct tgggaattta caattattcc ttacaatgga
      421 caaaaacatc aatctgatat cactgatatt gtaagtagtt tgcaattaca gttcgaatca
      481 tcggaagaag cagataaggg aaatagccac agtaaaaaaa tgcttaaagc acttctaagt
      541 gagggtgaaa gcatctggga gatcactgag aaaatactaa attcgtttga gtatacctcg
      601 agatttacaa aaacaaaaac tttataccaa ttcctcttcc tagctacttt catcaattgt
      661 ggaagattca gcgatattaa gaacgttgat ccgaaatcat ttaaattagt ccaaaataag
      721 tatctgggag taataatcca gtgtttagtg acagagacaa agacaagcgt tagtaggcac
      781 atatacttct ttagcgcaag gggtaggatc gatccacttg tatatttgga tgaatttttg
      841 aggaattctg aaccagtcct aaaacgagta aataggaccg gcaattcttc aagcaacaaa
      901 caggaatacc aattattaaa agataactta gtcagatcgt acaacaaggc tttgaagaaa
      961 aatgcgcctt atccaatctt tgctataaag aatggcccaa aatctcacat tggaagacat
     1021 ttgatgacct catttctgtc aatgaagggc ctaacggagt tgactaatgt tgtgggaaat
     1081 tggagcgata agcgtgcttc tgccgtggcc aggacaacgt atactcatca gataacagca
     1141 atacctgatc actacttcgc actagtttct cggtactatg catatgatcc aatatcaaag
     1201 gaaatgatag cattgaagga tgagactaat ccaattgagg agtggcagca tatagaacag
     1261 ctaaagggta gtgctgaagg aagcatacga taccccgcat ggaatgggat aatatcacag
     1321 gaggtactag actacctttc atcctacata aatagacgca ttctagaact atagtgagtc
     1381 gtattacgta gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     1441 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     1501 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     1561 tcagggggag gtgtgggagg ttttttaatt cgcggccgcg gcgccaatgc attgggcccg
     1621 gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     1681 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     1741 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     1801 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     1861 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     1921 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1981 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2041 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2101 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2161 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2221 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2281 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2341 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2401 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2461 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     2521 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2581 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2641 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2701 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2761 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2821 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2881 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2941 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     3001 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     3061 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     3121 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     3181 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     3241 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     3301 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     3361 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     3421 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3481 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3541 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3601 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3661 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3721 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3781 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
     3841 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     3901 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     3961 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     4021 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     4081 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     4141 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     4201 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     4261 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
     4321 ggcgatcggt gcgggcctct tcgctattac gccagtcgac catagccaat tcaatatggc
     4381 gtatatggac tcatgccaat tcaatatggt ggatctggac ctgtgccaat tcaatatggc
     4441 gtatatggac tcgtgccaat tcaatatggt ggatctggac cccagccaat tcaatatggc
     4501 ggacttggca ccatgccaat tcaatatggc ggacttggca ctgtgccaac tggggagggg
     4561 tctacttggc acggtgccaa gtttgaggag gggtcttggc cctgtgccaa gtccgccata
     4621 ttgaattggc atggtgccaa taatggcggc catattggct atatgccagg atcaatatat
     4681 aggcaatatc caatatggcc ctatgccaat atggctattg gccaggttca atactatgta
     4741 ttggccctat gccatatagt attccatata tgggttttcc tattgacgta gatagcccct
     4801 cccaatgggc ggtcccatat accatatatg gggcttccta ataccgccca tagccactcc
     4861 cccattgacg tcaatggtct ctatatatgg tctttcctat tgacgtcata tgggcggtcc
     4921 tattgacgta tatggcgcct cccccattga cgtcaattac ggtaaatggc ccgcctggct
     4981 caatgcccat tgacgtcaat aggaccaccc accattgacg tcaatgggat ggctcattgc
     5041 ccattcatat ccgttctcac gccccctatt gacgtcaatg acggtaaatg gcccacttgg
     5101 cagtacatca atatctatta atagtaactt ggcaagtaca ttactattgg aagtacgcca
     5161 gggtacattg gcagtactcc cattgacgtc aatggcggta aatggcccgc gatggctgcc
     5221 aagtacatcc ccattgacgt caatggggag gggcaatgac gcaaatgggc gttccattga
     5281 cgtaaatggg cggtaggcgt gcctaatggg aggtctatat aagcaatgct cgtttaggga
     5341 ac
//
